ORIGINAL_ARTICLE
Evaluation of the effect of tamoxifen citrate on model of osteoporosis in dog: biomechanical and histopathological studies
The effect of tamoxifen citrate on bone mass in immobilization osteoporosis was studied in 10 dogs.Osteoporosis was induced by fiberglass cast immobilization of the right hind-limb for 28 days, while the lefthind-limb served as a non-immobilized control. Five dogs received tamoxifen citrate (1.5 mg/kg per os) once daily for 28 days; five dogs received no treatment. All dogs were euthanized on day 28 and tibiae wereharvested. Bone biomechanical properties and microscopic structures of tibiae from casted and uncastedlimbs were studied. Significant differences in the percent of decreased values of examined mechanicalproperties were found between untreated and tamoxifen-treated dogs. No remarkable histopathologicalchanges indicative of osteoporosis were detected in the tibiae of casted limb of tamoxifen-treated dogs. These findings indicated that short term tamoxifen therapy may have promising effects on prevention ofosteoporosis in dog.
https://ijvr.shirazu.ac.ir/article_2641_90e851dbafeaa7369638282823b9db62.pdf
2006-09-01
1
5
10.22099/ijvr.2006.2641
Antiestrogen
Tamoxifen
Osteoporosis
Biomechanics
dog
S.
Saifzadeh
s.saifzadeh@mail.urmia.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
LEAD_AUTHOR
A.
Derakhshanfar
damin@mail.uk.ac.i
2
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
ORIGINAL_ARTICLE
Vitellogenin assay by enzyme-linked immunosorbent assay (ELISA) as a biomarker of endocrine disruptor chemicals (EDCs) pollution
There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially theestrogenic compounds. Work is ongoing to develop screening and testing programmes for endocrinedisrupting effects of new chemicals, and in the focus of this development are the fish test species commoncarp (Cyprinous carpio). In this study we have developed quantitative enzyme-linked immunosorbentassays (ELISA) for VTG in common carp. The working range of the ELISA was 11.25 to 2,000 ng/ml (25-75% specific binding/maximum antibody binding [B/B0]) with a 50% B/B0 intra- and interassay variationof 3.9% (n = 10) and 12.5% (n = 30), respectively. This ELISA is capable of detecting VTG as low as 6ng/ml, and can accurately detect VTG in even 10 μl of plasma. The ELISA was applied to measurement ofVTG production by male carp (Cyprinous carpio, Cyprinidae) fish exposed to ethynylestradiol. The resultsshowed that the amount of VTG produced in plasma of exposed fish increased in logarithmic ordercomparing to the control group and the ELISA described here could be used as an indicator of waterpollution to estrogenic pollutants.
https://ijvr.shirazu.ac.ir/article_2642_ee5b8193cd098b256723c799a24db4fa.pdf
2006-09-01
6
12
10.22099/ijvr.2006.2642
Immunoassay
ELISA
Endocrine disruptor chemicals
VTG
M.
Ebrahimi
ebrahimi@qom.ac.ir
1
Department of Physiology, Green Research Center, University of Qom, Qom, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Identification of excretory-secretory products from larval stages of Ostertagia circumcincta cultured in vitro by SDS-PAGE and immunoblotting
Exsheathing fluid (EF) and excretory-secretory products (ES) of infective third-stage cultured larvae ofOstertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE). Five and seven predominant proteins were found in the EF and ES products, respectively. Immunoblotting by sheep pre-infection serum did not react with any of the EF and ES proteins, but the postinfection serum recognized four proteins of 44.5, 41.5, 38 and 24 kDa of the ES products. None of the EF products was recognized by the post-infection serum. Protectivity of the four proteins remains to be determined.
https://ijvr.shirazu.ac.ir/article_2643_9fbefe637b2f2e45f2999d644a0471bf.pdf
2006-09-01
13
16
10.22099/ijvr.2006.2643
Ostertagia circumcincta
ES products
Sheep
SDS-PAGE
Immunoblotting
A. R.
Alborzi
alirezaalborzi@yahoo.com
1
Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
LEAD_AUTHOR
M. R.
Seyfiabad Shapouri
2
Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
N.
Hoghooghi Rad
3
Department of Parasitology, School of Veterinary Medicine Specialized Sciences, Islamic Azad University of Tehran, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Preliminary studies on reproductive activities of local Abadeh does, Fars province, southern Iran
No published information are available on the reproductive indices of local Abadeh does reared innorthern Fars province, southern Iran. This study was conducted to determine the duration of the breedingseason, and the length and duration of the oestrous cycle. Twenty single parity 2-year-old does from Abadeh(weighing 19–26 kg) were purchased and transferred to the School of Veterinary Medicine of University ofShiraz. They had access to alfalfa hay, water ad libitum and natural light. Vasectomised teaser buck wasturned with the flock and observation was made every 12 hrs during the day for standing heat detection, from late-August (2003) to late-August (2004). To determine the onset of reproductive activity as well asoccurrence of silent oestrus, blood samples were collected from jugular vein every 10 days. Following theonset of breeding season and observation of standing heat, 10 does were randomly selected in their 2ndoestrous cycle for twice weekly blood sampling. Serum progesterone concentration was determined using acommercial radioimmunoassay kit. It was found that silent oestrus occurs mostly in early breeding season.The first standing heat was observed in mid-September which was considered as the onset of breedingseason. Occurrence of standing oestrus increased through October, November and December. The peak ofstanding oestrus was recorded during late-October to early, mid-November. The mean ± SD duration of theoestrous cycle was 19.7 ± 1.1 days and the mean ± SD length of oestrus was 23.8 ± 12.3 hrs. The mean(±SD) concentrations of serum progesterone in days 0 (day of standing heat) to 4, varied between 0.1 and 0.8ng/ml. Concentrations of progesterone during the luteal phase varied between 2.7 and 3.9 ng/ml. The resultsof progesterone assay during oestrous cycle indicate that the follicular and luteal phases last about 4–5 daysand 14 days, respectively. In conclusion, oestrus activity in local Abadeh does is highly seasonal with a peakof activity being observed during the late-October to the end of November.
https://ijvr.shirazu.ac.ir/article_2644_19eeef77bf26cd196f1920dc36e321d8.pdf
2006-09-01
17
22
10.22099/ijvr.2006.2644
Abadeh does
Breeding season
Oestrous cycle
Standing heat
progesterone
M.
Emady
emadi21@yahoo.com
1
Department of Clinical Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
LEAD_AUTHOR
N.
Ahmadi
2
Department of Clinical Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
AUTHOR
M.
Kafi
3
Department of Clinical Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
AUTHOR
A.
Mirzaei
4
Department of Clinical Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
AUTHOR
ORIGINAL_ARTICLE
A comparison between the effects of a probiotic (Bioplus 2B) and an antibiotic (virginiamycin) on the performance of broiler chickens under heat stress condition
An experiment was conducted on 400 broilers to evaluate the effects of dietary supplementation ofantibiotic and probiotic on performance of birds under heat stress condition. The first group of chickens, thecontrol group, received a diet without any antibiotic and probiotic. The second group received diet with0.02% virginiamycin antibiotic; the two other groups were fed diets with 0.05% and 0.1% Bioplus 2Bprobiotic, respectively. The addition of antibiotic to the diet significantly improved body weight gain during0–3 and 4–6 weeks (heat stress) periods as compared to the control and 0.05% probiotic-supplementedgroups (P<0.05). Feed intake of different groups did not differ significantly (P>0.05). Addition of antibioticor various levels of probiotic to the diet significantly improved feed conversion ratio during 4–6 weeks (heatstress) period as compared to the control group (P<0.05). Supplementation of diet with antibiotic had nosignificant effect on antibody production against sheep red blood cells (SRBC), Newcastle disease virus(NDV) vaccine, white blood cell (WBC) count, heterophil to lymphocyte ratio (H/L), serum cholesterol andhaemoglobin content (P>0.05). Supplementation of diet with various levels of probiotic had no significanteffect on antibody titer against SRBC, NDV vaccine and haemoglobin content (P>0.05). However,supplementation of diet with 0.1% probiotic significantly increased WBC count and decreased H/L ascompared to the control group (P<0.05) which is important in reduction of stress effects on birds.
https://ijvr.shirazu.ac.ir/article_2645_d7784486e4f7418b9d4eee0b5733c05e.pdf
2006-09-01
23
28
10.22099/ijvr.2006.2645
Antibiotic
Probiotic
Performance
Broiler chickens
Sh.
Rahimi
rahimi_s80@yahoo.com
1
Department of Poultry Sciences, College of Agriculture, Tarbiat Modarres University of Tehran, Tehran, Iran
LEAD_AUTHOR
A.
Khaksefidi
2
Department of Poultry Sciences, College of Agriculture, Tarbiat Modarres University of Tehran, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Cadmium as an etiology of sperm dysfunction in Holstein bulls
Oxidative stress has been identified as a crucial factor leading to male infertility largely due to peroxidativedamage to the sperm cell membrane. Cadmium (Cd) is a widespread environmental pollutant and oneof the well-known reproductive toxicants. Because of its long biological half-life (10–30 years), Cdaccumulates in the biological systems. The present study was designed to assess the concentration-dependent in vitro effect of 20 to 700 μM of Cd on the membrane integrity, motility, and acrosomal status of Holstein bull spermatozoa. We recorded a significant elevation in the malondialdehyde (MDA) level, increased lipid peroxidation (LPO) rate and a drastic decrease in the spermatocrit values, especially at the 700 μM concentration of Cd, indicating deleterious effects of Cd on the intactness of the sperm membrane. There was also a negative correlation between the LPO rate and both the percentage of motile spermatozoa (r = -0.89) and sperm viability (r = -0.86). Performing the gelatin test indicated that Cd altered the integrity of acrosomal membranes and showed an abnormal acrosome reaction. In this regard, a reverse correlation was found between the LPO rate and the percentage of halos (r = -0.96). In conclusion, Cd was proved to be a potential toxicant in the category of environmental factors that induced membrane impairments, lowered motility and viability, and decreased rate of acrosome reactions leading to bull sperm dysfunction.
https://ijvr.shirazu.ac.ir/article_2646_1145e603ef436aeec8e486ad1f798e6c.pdf
2006-09-01
29
36
10.22099/ijvr.2006.2646
cadmium
Bull sperm
Membrane integrity
Motility
Acrosome
M.
Arabi
mehranarabi@sci.sku.ac.ir
1
Department of Biology, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Pathogenicity and haemagglutinin gene sequence analysis of Iranian avian influenza H9N2 viruses isolated during (1998–2001)
Sixteen avian influenza (AI) H9N2 viruses were isolated from disease outbreaks in different parts of Iranduring (1998–2001). These AI isolates were used for pathogenicity, haemagglutinin (HA) gene variation andphylogenetic analysis. Results in both pathogenicity tests and HA gene cleavage site sequence detectionrepresented a non-highly pathogenic feature for all Iranian AI isolates studied. The cleavage site motif (R-SS-R) of all AI isolates however, indicated that they had capability of becoming highly pathogenic virusesfollowing 2 nucleotide substitutions at this region. Based on 450 nucleotides region obtained for localisolates and those for referenced viruses available in Gene Bank database used in phylogenetic analysis, allviruses placed on 3 distinct groups, 2 for Iranian and 1 for reference viruses. Among the reference AI viruses, isolates from Pakistan, Saudi Arabia and 1 from Germany showed less differences with Iranian AI isolates. Results also revealed that the circulating viruses in neighbouring provinces have been remained with less mutation for about 2 years.
https://ijvr.shirazu.ac.ir/article_2647_86d0496df69e9ff4bff7394d22a08b00.pdf
2006-09-01
37
41
10.22099/ijvr.2006.2647
Influenza H9N2 virus
Iranian
pathogenicity
Phylogenetic
M.
Kianizadeh
kiian@imamreza.net
1
Department of Veterinary Research and Biotechnology, Razi Vaccine and Serum Research Institute, Mashhad, Iran
LEAD_AUTHOR
S. A.
Pourbakhsh
2
Department of Research and Diagnosis of Avian Diseases, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR
R.
Toroghi
3
Department of Veterinary Research and Biotechnology, Razi Vaccine and Serum Research Institute, Mashhad, Iran
AUTHOR
R.
Momayez
4
Department of Research and Diagnosis of Avian Diseases, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR
ORIGINAL_ARTICLE
Seasonal changes of pronephros lymphoid tissue in grass carp (Ctenopharingodon idella): a histometrical and histological study
The major lymphoid tissues in teleost fish are the kidneys, thymus, spleen and mucosa-associatedlymphoid tissue including the skin, gills and intestine. The head of kidney (pronephros) is an importanthaematopoietic organ and has morphological similarities with the bone marrow in higher vertebrates. In thisstudy, during 12 months from August 2002 to July 2003, 120 grass carp (10 fish/month) were harvested from 3 fish culture ponds in Ahvaz. The water, weather temperatures and light duration of days (photoperiod) were recorded. After biometrical examination of age, weight and the length of the fishes, the whole kidneys were removed from abdominal cavity and samples were fixed in bouin’s solution for light microscopy and in glutaraldehyde for electron microscopy. The results showed that the lymphoid tissue distribution has significant changes during different seasons so that, the mean ± SEM of lymphoid tissue distribution in hot seasons (33.53 ± 0.59 mm2/cm2) was more than cold seasons (19.20 ± 0.65 mm2/cm2). Also the statistical results showed that water temperature has more significant (P<0.05) effect on lymphoid tissue. Degenerated lymphoid cells with pale and vacuolated cytoplasm were observed. A reversed correlation was found between the number of normal and degenerated lymphocytes. Electron microscopy revealed that degenerated lymphocytes were devoid of membranous organelles, their cytoplasms were vacuolated and the nuclear envelope had some alterations.
https://ijvr.shirazu.ac.ir/article_2648_00eb7316897cb2a17095b2a029e996e3.pdf
2006-09-01
42
49
10.22099/ijvr.2006.2648
Seasonal changes
Pronephros
Lymphoid tissue
Grass carp
H.
Morovvati
hmorovvati@scu.ac.ir
1
Department of Histology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
LEAD_AUTHOR
N.
Alboghobeish
2
Department of Histology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
A.
Noori
3
Head of Ultrastructure Center, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR
A.
Rasekh
4
Department of Statistics, Faculty of Computer and Mathematic Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
ORIGINAL_ARTICLE
Polymerase chain reaction typing of Pasteurella multocida capsules isolated in Iran
Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. multocida isolates belonged tocapsular type A. The sequence alignment of the fragment showed a high similarity (>96%) with thepublished sequences of P. multocida hya gene in the Gene Bank. It was recognised that P. multocida capsulargroup A is the dominant cause of fowl cholera in Iran.
https://ijvr.shirazu.ac.ir/article_2649_272739622056ade4f6ec88f23c87a26f.pdf
2006-09-01
50
55
10.22099/ijvr.2006.2649
P. multocida
Capsule
PCR
Typing
A. R.
Jabbari
ahmadjb@yahoo.com
1
Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran
LEAD_AUTHOR
M.
Esmaelzadeh
2
Department of Biotechnology, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR
Gh. R.
Moazeni Jula
3
1Department of Veterinary Aerobic Bacterial Vaccines, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR
ORIGINAL_ARTICLE
Immunization of lambs with whole body Echinococcus granulosus
We conducted this study to determine the level of immunity after vaccination of lambs with whole bodyEchinococcus granulosus. To do so, 200 mature E. granulosus parasites, which were kept in 10% formalinefor 8 months, were obtained from the Department of Parasitology, School of Veterinary Medicine, FerdowsiUniversity of Mashhad. The soluble protein of the parasite was prepared. The sample was homogenized in ablender, sonicated on ice and then centrifugated for 15 min at 10,000 g. Final yield was kept at -20ºC untilused. Eight 4–6-month-old lambs of mixed sex, were divided into 2 equal groups; each lamb in the test groupwas vaccinated subcutaneously in the neck with 2 ml of the vaccine (1 mg of whole body of E. granulosusprotein dissolved in 1 ml of PBS plus 1 ml of Freund’s complete adjuvant (FCA)). The control lambs werevaccinated only with adjuvant in PBS. Lambs were re-vaccinated four weeks after the first vaccination withthe same preparation except that FCA was replaced by Freund’s incomplete adjuvant (FIA). Three weekslater, each lamb was administered a challenge infection dose of 2000 protoscolices intraperitoneally and 10adult E. granulosus. After 7 months, all lambs were killed and examined for hydatid cysts. We found twocysts in the liver and and one in the lung of only one of the vaccinated lambs. The number of cysts invaccinated lambs were significantly lower than that in the control group (P<0.001). This means that theprotective immunity in lambs with whole body of E. granulosus was approximately 90%.
https://ijvr.shirazu.ac.ir/article_2650_f731c78e80e2e73cd8e38a110059d83e.pdf
2006-09-01
56
59
10.22099/ijvr.2006.2650
Echinococcus granulosus
Whole body
Vaccination
Lamb
G. R.
Hashemitabar
hashemit@um.ac.ir
1
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
G. R.
Razmi
razmi@ferdowsi.um.ac.ir
2
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
A.
Naghibi
3
Department of Pathobiology, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
ORIGINAL_ARTICLE
Veterinarians and dairy nutrition management: basic concepts and design-it-yourself—a veterinary-oriented ration evaluation program
Veterinarians increasingly play a major contributory role in dairy practice and management. Withincreasing importance of economical pressures, bioenvironmental limits and increasing demand for higherquality products, veterinarians are more involved in nutrition management and advice, both from aneconomical view of productivity and preventive aspects of veterinary medicine. The concept of food animalproduction medicine is the hallmark of such a change from purely diagnosis and treating sick cows to designstrategies for disease prevention and economical profitability. One of the essential parts of the new role is tohave a good command of nutritional concepts and acquaintance with ration formulation procedures and alsosoftwares. It appears that the available ration formulation softwares are insufficient in view of estimatingnecessary criteria for dairy practitioners. Moreover, the release of new edition of NRC and subsequentmodification of software programs for ration formulation always lag behind the generation of newknowledge in dairy nutrition. This article describes the basic nutritional concepts, as well as a practicalapproach to design a ration evaluation program based on Microsoft® Excel. The program can be designed in a profession-oriented approach.
https://ijvr.shirazu.ac.ir/article_2651_f6b32cf4c7d25b50049b2b43de03be2d.pdf
2006-09-01
60
67
10.22099/ijvr.2006.2651
Spread sheet
Ration evaluation
Dairy cattle
K.
Sharifi
shariffp@ferdowsi.um.ac.ir
1
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Epidemiology of Paramphistomiasis in buffaloes under different managemental conditions at four districts of Punjab province, Pakistan
Epidemiological studies were undertaken at slaughter-houses, livestock farms, veterinary hospitals andon household buffaloes under climatic conditions of four different districts of Punjab province. Infection ratewas 22.29, 28.33, 17.08 and 12.75%, respectively in slaughter-house buffaloes, livestock farm buffaloes,veterinary hospital buffaloes and household buffaloes. Overall the season wise the highest prevalence(28.33%) was recorded in buffaloes at livestock farms followed by slaughtered (22.29%) and veterinaryhospital buffaloes (17.08%). While the lowest (12.75%) prevalence was recorded in household buffaloesduring spring. It was also observed that the higher infection rate was recorded in younger buffaloes (belowtwo years of age) than older (above two years of age) where as sex wise the prevalence indicated that malebuffaloes were more commonly affected than females. Snails belonging to genera Bulinus, Lymnaea andPlanorbis were also observed which are responsible for the transmission of paramphistomiasis.
https://ijvr.shirazu.ac.ir/article_2652_04e0bc62aff87324d4d19c09236db53b.pdf
2006-09-01
68
72
10.22099/ijvr.2006.2652
Epidemiology
Paramphistomiasis
Buffaloes
Punjab
Pakistan
U.
Javed Khan
1
Department of Zoology, University of Punjab, Lahore, Pakistan
AUTHOR
T.
Akhtar
2
Department of Zoology, University of Punjab, Lahore, Pakistan
AUTHOR
A.
Maqbool
azhar2003m@yahoo.com
3
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan
LEAD_AUTHOR
A.
Anees
4
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore, Pakistan
AUTHOR
ORIGINAL_ARTICLE
Comparison of Dot-ELISA with microbial culture for detection of Brucella spp. in clinical specimens
Definitive diagnosis of brucellosis is made by isolation of the causative agents, which is a timeconsumingprocedure. To evaluate the efficacy of Dot-ELISA for detecting brucellae in clinical samples, 94different specimens taken from animal origin were cultured on brucella selective culture media and colonieswere identified biochemically. The specimens were also examined after centrifugation by Dot-ELISA using aspecific anti-brucella antibody, a suitable peroxidase conjugate and substrate. Of the 94 samples, 5 (5.31%)were positive in Dot-ELISA and 4 (4.25%) had positive cultures. In comparison with culture, the sensitivityand specificity of Dot-ELISA for detection of brucellae in the samples was 80 and 100%, respectively. Therewas 98.9% agreement between the two tests. The results indicated that Dot-ELISA is a good and rapid testwith acceptable sensitivity and specificity for detection of Brucella spp. in aborted fetal stomach contents.
https://ijvr.shirazu.ac.ir/article_2653_f62a0bf466a8f7a826ea25e2105277f4.pdf
2006-09-01
73
75
10.22099/ijvr.2006.2653
Brucellosis
Brucella spp
Dot-ELISA
M.
Ghorbanpoor
ghorbanpoor2000@yahoo.com
1
Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
LEAD_AUTHOR
M. R.
Seyfiabad Shapouri
2
Department of Pathobiology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
S.
Goraninejad
3
Department of Clinical Sciences, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
E.
Jalali
4
Graduated from School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
ORIGINAL_ARTICLE
Prevalence of bacterial mastitis in cattle from the farms around Tehran
To determine the prevalence of bacterial mastitis in cattle, milk samples positive for California mastitistest (CMT) were cultured during a period of almost 4 years. The bacterial species isolated from 2904 milksamples studied were coagulase negative Staphylococcus sp. in 879 (30.27%) samples, Streptococcusagalactiae in 642 (22.11%), S. dysgalactiae in 332 (11.43%), E. coli in 295 (10.16%), Staphylococcus aureusin 84 (2.89%), Bacillus cereus in 51 (1.76%), Arcanobacterium pyogenes in 31 (1.07%), Pseudomonasaeruginosa in 6 (0.21%), Klebsiella pneumoniae in 4 (0.14%), Pasteurella multocida in 1 (0.03%) andMycoplasma sp. in another (0.03%) sample. No growth was found in 578 samples (19.90%). Thirty-one(37%) of 84 animals which were infected with S. aureus, had acute infection. We found that contaminationof milk with coagulase negative staphylococci are the most frequent bacterial infection in dairy cattle aroundTehran; it mostly causes subacute form of the disease. S. agalactiae, S. dysgalactiae and E. coli are thesecond, third and the fourth causative agents.
https://ijvr.shirazu.ac.ir/article_2654_2f42d7223248d81c919972a322081a2b.pdf
2006-09-01
76
79
10.22099/ijvr.2006.2654
Dairy cattle
Mastitis
Pathogenic bacteria
Acute infection
Subacute infection
N.
Atyabi
natyabi@chamran.ut.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
M.
Vodjgani
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
F.
Gharagozloo
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Bahonar
4
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Seroepidemiology of Newcastle disease in domestic village chickens of plain areas of Isfahan province, central Iran
Newcastle disease is one of the most important diseases of poultry. It usually causes a great loss inpoultry industry and domestic village chickens. Since domestic chickens in villages are free ranging for food,the chance of their contact with wild birds that may act as reservoirs is high. To determine the role ofdomestic chickens in the epizootiology of Newcastle disease virus in villages of Isfahan province, 400 serumsamples from chickens with no history of vaccination from four regions (Khomeinishahr, Zarinshahr,Falavarjan and Mobarekeh) were collected in summers of (1998) and (1999). Haemagglutination inhibition(HI) test was used for titration of antibodies against Newcastle disease virus. Chi-square and binomial testswere used for statistical analyses. 69.5 and 68.5% of the sera were positive in the two consecutive seasons(P>0.05). About 25% of 3–4 month- and 1–2-year-old chickens were negative for HI antibodies and thuswere sensitive to the disease in each season. Significant correlation was observed between the HI antibodytiters and the age of the chickens (Pindependent to the prevalence of the disease in industrial poultry flocks in each region and all regionsstudied. It was concluded that about 35% of the domestic village chickens are protected against virulentstrains of Newcastle disease in summer. For protection of the remaining chickens, routine vaccination,especially in spring and summer is suggested.
https://ijvr.shirazu.ac.ir/article_2655_3f70f8d272cc8a7bc11fce16fdb6e71f.pdf
2006-09-01
80
84
10.22099/ijvr.2006.2655
Domestic village chickens
Seroepidemiology
Newcastle disease
Iran
M.
Bouzari
bouzari@sci.ui.ac.ir
1
Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
LEAD_AUTHOR
R.
Mousavi Morekani
2
Isfahan Veterinary Network, Isfahan, Iran
AUTHOR
ORIGINAL_ARTICLE
Serologic evidence of bluetongue infection in onehumped camels (Camelus dromedarius) in Kerman province, Iran
Herein, we presented the first report on bluetongue (BT) disease in 10 pregnant camels in a herd fromKerman province, Iran. All sera samples were tested serologically (AGID, C-ELISA). We also used the Razi-BK cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ECE) to culture and isolate the BT virus. Efforts to culture and isolation of BT virus have metwith very limited success. Following precipitation test (AGID) and C-ELISA, 5 of 10 sera in AGID test, andall in C-ELISA became positive. Further studies are needed on the ecology of camels and vector midges toclarify the reason for infection of the camels in Iran.
https://ijvr.shirazu.ac.ir/article_2656_bb2952b5befa9444d406a49848c9549e.pdf
2006-09-01
85
87
10.22099/ijvr.2006.2656
Bluetongue
Serology
ELISA
CAMELS
S.
Mahdavi
s.mahdavi@rvsri.com
1
Department of Virology, Razi Vaccine and Serum Research Institute, Karadj, Iran
LEAD_AUTHOR
K.
Khedmati
2
Department of Virology, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR
L.
Pishraft Sabet
3
Department of Virology, Razi Vaccine and Serum Research Institute, Karadj, Iran
AUTHOR