ORIGINAL_ARTICLE
Expression and analysis of the complement resistant trait Iss, from E. coli strain χ1378 isolated from poultry colibacillosis in Iran
Avian pathogenic E. coli (APEC) is responsible for economic losses in all poultry farms. Certain virulence factors have been proposed as a means of controlling APEC infections, including some proteins to be used for vaccination. In the study we report here, one of the major virulence factors, the iss (increased serum survival) gene, from E. coli strain χ1378, isolated from poultry colibacillosis in Iran, was cloned to construct a prokaryotic expression vector, in order to analyse the Iss protein. The iss gene was successfully cloned into the pGEX-3X vector. The construct was transformed into E. coli BL21 to express the Iss protein under induction. The Iss protein was expressed as a glutathione-S-transferase (GST) fusion protein. GST::Issprotein was sequenced by MS/MS MALDI-TOF techniques to confirm its amino acid sequence. BLAST analysis of the Iss protein showed high similarity with previously submitted sequences. Overall, it seems that the Iss protein from strain χ1378 could be used as a good antigen to vaccinate against poultry colibacillosis.GST::Iss protein is currently being used as recombinant protein in SPF chicken models with the goal ofevaluating the immune response for APEC control. In conclusion, we constructed a prokaryotic expressionvector of the iss gene, and express and sequence the Iss protein from E. coli strain χ1378 isolated fromsystemic colibacillosis.
https://ijvr.shirazu.ac.ir/article_78_27cf6c7e665de0b3b58d2358b59f2d5d.pdf
2011-12-01
283
291
10.22099/ijvr.2011.78
Iss protein
Avian pathogenic E. coli
Expression
Vaccination
A.
Derakhshandeh
1
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
T.
Zahraei Salehi
2
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
M.
Muniesa
mmuniesa@ub.edu
3
Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain
LEAD_AUTHOR
ORIGINAL_ARTICLE
Evaluation of pharmacological mechanisms of antinociceptive effect of Teucrium polium on visceral pain in mice
Teucrium polium is used for treatment of visceral pain in Iranian folkloric medicine. In this studyantinociceptive mechanisms of T. polium hydroethanolic extract were examined by acetic acid-inducedwrithing test as a model of visceral pain in male NMRI mice. To reveal the antinociceptive mechanisms ofthe extract, we examined the effects of opioidergic, serotonergic, adrenergic and histaminergic antagonists on extract-induced antinociception. The results of this study showed that pretreatment with naloxone,chlorpheniramine and cimetidine significantly attenuate the antinociceptive effect of the extract. However,cyproheptadine and phentolamine had no effect. Our results clearly show antinociceptive effects of T. poliummay be mediated by opioidergic and histaminegic H1 and H2 receptors.
https://ijvr.shirazu.ac.ir/article_79_ca068802e1cfe1d6e3d7c8fd7c548b0d.pdf
2011-12-01
292
297
10.22099/ijvr.2011.79
Teucrium polium
Opioid receptor
Histamine receptors
Writhing test
Mouse
M.
Zendehdel
zendedel@ut.ac.ir
1
Department of Physiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
M.
Taati
Taatim@lu.ac.ir
2
Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran
AUTHOR
M.
Jadidoleslami
3
Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Bashiri Dezfouli
4
DVM Student, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Effects of latanoprost and pilocarpine combination on the intraocular pressure and pupil size of normal rabbits
The aim of this study was to determine the combination effect of latanoprost and pilocarpine on theintraocular pressure and pupil size in normal rabbits. In this study, 18 rabbits were randomized to threegroups of 6 animals each. The right eyes of rabbits in group 1 were treated topically with latanoprost, ingroup 2 with pilocarpine and in group 3 with latanoprost and pilocarpine. The left eyes received placebo.Drugs were instilled once a day at 8 am over 4 days. IOP and pupil diameter measurements were made at 8am, 10 am, 12 noon, 2 pm and 4 pm during the 4 days of treatment, the 2 days that preceded treatment, and 3 days following treatment. The occurrence of blepharospasm and conjunctival hyperemia were also evaluated at the same times that the measurements were made. The mean IOPs were significantly lower than the contralateral eyes in 8 of the 20 time intervals (40%) in both latanoprost and pilocarpine-treated and in 18 of 20 time intervals (90%) in latanoprost plus pilocarpine-treated eyes in the treatment period. The mean daily hypotensive effects of latanoprost, pilocarpine and their combination were 4.5 (31%), 2 (14.4%) and 5 mmHg (34.7%), respectively. Although the mean IOPs in group 3 have decreased more than other groups, the differences between the three groups are not significant. Conjunctival hyperemia was observed in the treated eyes of the three groups. It is concluded that topical instillation of the combination of latanoprost and pilocarpine was not as effective in IOP reduction than by drugs alone and that hyperemia is the most frequent side effect observed during the treatment period.
https://ijvr.shirazu.ac.ir/article_80_18a4bac5081054a74fb18c5598d7782e.pdf
2011-12-01
298
303
10.22099/ijvr.2011.80
Intraocular pressure
Latanoprost
Pilocarpine
Pupil diameter
Rabbit
A. A.
Sarchahi
sarchahi@um.ac.ir
1
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
M. A.
Gholipour
2
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
F. S.
Toghraie
3
Graduated from School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
ORIGINAL_ARTICLE
Morphological and histochemical investigation of the camel (Camelus dromedarius) abomasal mucous membrane by light and scanning electron microscopy (SEM)
Morphological and histochemical study of the abomasal epithelium in camel (Camelus dromedarius) wascarried out by light and scanning electron microscopes. The lining of the abomasum was divided into fourregions, i.e. cardiac, pseudocardiac, fundic and pyloric. Our investigation revealed that the cardiac andpseudocardiac regions occupy a wide part of the abomasum in camel and it reaches approximately the thirdfourth of the abomasum. Our gross anatomical observation showed small diverticulae in the Fundic region, this part was covered with thick mucosal folds that have been separated by deep branching furrows. In the histological study we observed that, the mucosa has extensive gastric folds and small invaginations or gastric pits, which were in continuation with the gastric glands. The mucosal surface was covered with simple columnar epithelial. Our histochemical revealed that the surface epithelium in abomasum was negative to AB and positive to PAS staining, whereas in the gastric pit cells it was positive to AB and PAS staining, but gastric gland cells were negative to PAS and positive to AB staining. In the SEM study, after complete removal of mucin from the surface of the mucosa, simple columnar epithelial cells with a mean length of 20 μm were observed, and some epithelial cells have been arranged as flower body (FB). Also, we observed hexagonal structures on the surface of the abomasums that resembled honeycomb structure (HC). Mean diameter of these HC structures was 30-40 μm. For the first time our investigation revealed these FB and HC structures in the abomasal mucosa of camel.
https://ijvr.shirazu.ac.ir/article_81_d57dbe46826a5a57d4769c8819eb23af.pdf
2011-12-01
304
308
10.22099/ijvr.2011.81
Histomorphology
Histochemistry
Abomasum
Camel
SEM
A. R.
Raji
Rajireza@ferdowsi.um.ac.ir
1
Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Genotyping of Fusarium verticillioides strains producing fumonisin B1 in feed associated with animal health problems
Fusarium verticillioides (F. verticillioides) is not only a primary pathogen of maize, but also can causedisease in other crops such as sorghum. Pathogenicity is related to mycotoxin production such as fumonisin.In the present study, 24 isolates of F. verticillioides, which were previously identified by phenotype basedmethods, were re-identified using restriction fragment length polymorphism (RFLP) analysis. Digestion ofthe polymerase chain reaction (PCR) products with the restriction enzyme TasI allowed identifying four nonverticillioides strains that were discarded from our study. The genetic variations among the remaining 20strains of F. verticillioides were analysed by random amplified polymorphic DNA (RAPD)-PCR methodwith 4 primers. Of the four primers tested, two primers produced polymorphic amplification patterns.Dendrogram for each primer indicated the distance of the strains to each other. Using primers of A, B, C andD, the isolates were divided to 8, 9, 7 and 7 groups, respectively. The results of this study indicated geneticrelationship among DNA polymorphic patterns with geographic regions and the severity of fumonisin B1(FB1) production. It seems that RAPD analysis is a suitable technique for strain typing of F. verticillioides.
https://ijvr.shirazu.ac.ir/article_82_8c2c77e6c7bb20d9e9af05ff63d1a5d9.pdf
2011-12-01
309
316
10.22099/ijvr.2011.82
Fusarium verticillioides
Genotyping
RAPD-PCR
Maize
R.
Daie Ghazvini
1
Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
H.
Mirhendi
2
Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
S. A.
Ghiasian
3
Faculty of Medicine, Hamedan University of Medical Sciences, Hamedan, Iran
AUTHOR
A.
Masoudi-Nejad
4
Laboratory of Bioinformatic, Biophysics-Biochemistry Research Center, University of Tehran, Tehran, Iran
AUTHOR
H.
Shokri
5
Faculty of Veterinary Medicine, University of Mazandaran, Amol, Iran
AUTHOR
M.
Soltani
6
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
S.
Haddadi
7
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A. R.
Khosravi
Khosravi@ut.ac.ir
8
Mycology Research Center, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
The in vitro effects of nanosilver colloid on kinematic parameters of ram spermatozoa
This study investigated the concentration/time dependent effects of nanosilver colloid on the motionparameters of the ejaculated ram spermatozoa in vitro. After incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. There was a significant (P<0.05) decrease in most motion of sperm parameters (the fast, slow and non-progressive motility, VCL, VSL, STR, MAD, ALH, WOB, VAP and LIN) in treated groups compared to their corresponding controls, especially after 120 min of incubation. On the other hand, there was a significant (P<0.05) increase in sperm immotility in comparison with the control group in all times at 1 ppm nanosilver colloid. At a concentration of 10 ppm, spermatozoa were completely inactivated. It is concluded that nanosilver colloid depresses sperm functions, especially motility parameters, which can be a causative agent for sperm infertility induced by nanosilver cytotoxicity.This study investigated the concentration/time dependent effects of nanosilver colloid on the motion parameters of the ejaculated ram spermatozoa in vitro. After incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. There was a significant (P<0.05) decrease in most motion of sperm parameters (the fast, slow and non-progressive motility, VCL, VSL, STR, MAD, ALH, WOB, VAP and LIN) in treated groups compared to their corresponding controls, especially after 120 min of incubation. On the other hand, there was a significant (P<0.05) increase in sperm immotility in comparison with the control group in all times at 1 ppm nanosilver colloid. At a concentration of 10 ppm, spermatozoa were completely inactivated. It is concluded that nanosilver colloid depresses sperm functions, especially motility parameters, which can be a causative agent for sperm infertility induced by nanosilver cytotoxicity.
https://ijvr.shirazu.ac.ir/article_83_b2c0567ee08061149f3bed1c5e94f89c.pdf
2011-12-01
317
323
10.22099/ijvr.2011.83
Nanosilver colloid
Ram
Ejaculated sperm
Motion parameters
P.
Mirshokraei
mirshokraei@vet.sku.ac.ir
1
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
LEAD_AUTHOR
H.
Hassanpour
2
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
AUTHOR
M.
Akhavan Taheri
3
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
AUTHOR
M.
Riyahi
4
MSc Student in Animal Physiology Science, Department of Animal Sciences, Faculty of Agriculture, Zanjan University, Zanjan, Iran
AUTHOR
N.
Shams-Esfandabadi
5
Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
AUTHOR
ORIGINAL_ARTICLE
Phylogenetic analysis of PB2 gene of H9N2 subtype of avian influenza viruses isolated from commercial chickens in Tehran province of Iran during 1998-2001
The PB2 protein of influenza A viruses has been shown as host range determinant. In this study PB2gene of five H9N2 isolates from Tehran province during the period of 1998-2001 were partially amplifiedand sequenced to do phylogenetic study. Iranian isolates could be classified in Eurasian lineage according totheir PB2 gene sequences. The PB2 genes of these isolates are different from those of Middle-East countriessuch as the UAE, Pakistan and Saudi Arabia, as well as India, and it is probable that their ancestors haveundergone reassortment with other subtypes. The Nucleotide sequences of their PB2 are highly similar toeach other with 97.5-99.6% homology percentage showing the least mutations in their genes during theperiod of 1998 to 2001. The PB2 of the Iranian isolates contain Glutamic acid-(E) in position 627, Asparticacid-(D) in 701 and Serine-(S) in 714 as other avian isolates.
https://ijvr.shirazu.ac.ir/article_84_f7ff368b667f71f8e80a6eaefb2e5465.pdf
2011-12-01
324
331
10.22099/ijvr.2011.84
Avian influenza
H9N2
PB2
Phylogenetic analysis
J
Pazani
pazani@avianclinic.com
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
V.
Karimi
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
M. H.
Bozorgmehri Fard
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Ghalyanchi Langeroudi
4
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Barin
5
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Effects of aflatoxin B1 on profiles of gonadotropic (FSH and LH), steroid (testosterone and 17β-estradiol) and prolactin hormones in adult male rat
Aflatoxins (AFs) are natural contaminants of feed and feedstuffs, thus the study of the noxious effects ofthese agents on the male reproductive system is of outstanding importance. Our aim for this study is theevaluation of the effects of oral administration of aflatoxin B1 on the reproductive hormonal changes in adult male rat. Twenty eight Wistar strain male rats were selected for this study. The rats were divided into 4groups, viz, control (C), test groups (T1, T2, and T3). The toxin doses were prepared in sterile distilled waterat 0.8 ppm, 1.6 ppm, 3.2 ppm, and administered through oral gavages, 1 ml/animal/day to groups T1, T2, and T3, respectively for 48 days and each animal of group C was gavaged with 1 ml/day sterile distilled water. The hormonal assays were carried out using ELISA biochemical kits for serum FSH, LH, prolactintestosterone and 17β-estradiol. Results showed that, the levels of serum LH and testosterone were lower(P<0.001), but conversely the levels of FSH and prolactin were higher (P<0.001) in the test groups. The levelof 17β-estradiol was affected by significantly falling (P<0.01) only in group T3. We conclude that, the oraladministration of the aflatoxin B1 strictly alters the concentrations of FSH, LH, prolactin, and testosterone inmale Wistar rats.
https://ijvr.shirazu.ac.ir/article_85_7fac07067a567ac2e94a0dfdd012c341.pdf
2011-12-01
332
336
10.22099/ijvr.2011.85
Male rat
aflatoxin B1
Pituitary hormones
Testosterone
17β-estradiol
Sh.
Hasanzadeh
s.hasanzadeh@mail.urmia.ac.ir
1
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
LEAD_AUTHOR
E.
Hosseini
2
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
L.
Rezazadeh
3
Graduated from Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
ORIGINAL_ARTICLE
A serological survey on leptospirosis in aborted dairy cattle in industrial farms of Hamedan suburb, Iran
Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans. It is a well known cause of bovine reproductive losses such as abortion, infertility, stillbirth and birth of weak calves. Inthis research, the relationship between the seroprevalence rate of Leptospira spp. infection and abortion inindustrial dairy farms of Hamedan province, Iran was studied. A total of 80 blood samples were taken fromaborted cows in six dairy farms. Sera were tested for antibodies against 6 serovars of Leptospira interrogans(hardjo, pomona, canicola, grippotyphosa, icterohaemorrhagiae and ballum) using microscopic agglutination test (MAT). Antibodies were detected in 18 (22.5%) of the aborted cows, including 17 (21.25%) against L. canicola and 1 (1.25%) against L. pomona. It is concluded that dogs (shepherd and stray) and wild carnivores may have an important role to maintain and transmit the L. canicola infection to the cattle population in this region, therefore, vaccination of cattle and shepherd dogs should be applied.
https://ijvr.shirazu.ac.ir/article_86_227f42019294e2de14317f4952d2ce1b.pdf
2011-12-01
337
339
10.22099/ijvr.2011.86
Leptospirosis
Abortion
Cattle
Microagglutination test
A.
Bahari
aliasghar.bahari@basu.ac.ir
1
Department of Veterinary Medicine, Faculty of Paraveterinary Sciences, Bu-Ali Sina University, Hamedan, Iran
LEAD_AUTHOR
G.
Abdollahpour
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Sadeghi-Nasab
3
Department of Veterinary Medicine, Faculty of Paraveterinary Sciences, Bu-Ali Sina University, Hamedan, Iran
AUTHOR
S.
Sattari Tabrizi
4
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
M.
Yavari
5
Department of Veterinary Medicine, Faculty of Paraveterinary Sciences, Bu-Ali Sina University, Hamedan, Iran
AUTHOR
B.
Dadmehr
6
Applied Science Education Center of Amarlou, Jirandeh, Iran
AUTHOR
ORIGINAL_ARTICLE
Survey of canine Dirofilaria immitis infection in Caspian provinces of Iran
Between July and August 2009, a total of two hundred crossbreed dogs in three contiguous littoralprovinces of Guilan, Mazandaran and Golestan were sampled. Circulating antigens and/or microfilariae ofDirofilaria immitis were found in 25.5% of dogs in the whole studied area. The highest infection rate wasfound in Guilan (51.42%), followed by Golestan and Mazandaran provinces (15.38% and 7.69%, respectively). Prevalence values were not influenced by age and sex in the whole area; the values detected in low altitude regions were 4.6 times the values found in high altitude areas. This is the first detailed survey on canine dirofilariasis in these coastal provinces of Iran. Guilan province, with its certain hyper endemiccounties, was shown to be the most endemic for canine heartworm infection in the country. hemoprophylaxis should be considered for the dog population in these areas.
https://ijvr.shirazu.ac.ir/article_87_1046a8d2ab280be79fb461672672ea67.pdf
2011-12-01
340
344
10.22099/ijvr.2011.87
Dirofilaria immitis
Heartworm
Canine
Caspian provinces
Iran
A.
Malmasi
amalmasi@ut.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
S. H.
Hosseini
hhoseini@ut.ac.ir
2
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
M.
Aramoon
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
A.
Bahonar
4
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
H. A.
Seifi
5
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
ORIGINAL_ARTICLE
Isolation, identification and antibiotic sensitivity determination of Ornithobacterium rhinotracheale in
slaughtering broiler chicken flocks of Guilan province
Ornithobacterium rhinotracheale (ORT), a species of bacteria, is known to be associated with respiratorydisease, growth retardation, mortality and decreased egg production. The aim of the present study was todetermine seroprevalence of the infection and to identify the microorganism by bacteriological and molecularmethods. In this study, 460 serum samples and also 290 tracheal swabs were collected from 29 broilerchicken flocks in slaughter houses. Serological results showed that 24 out of 460 (5.12%) serum samplesrelated to 5 flocks were positive for ORT by ELISA test. ORT was isolated from tracheal swabs of 3 flocks(1.03% out of 290 tracheal swabs) by culture and confirmed as ORT by specific primers in PCR method.Statistically, there was no significant difference between the rate of isolation and ORT serum titers (P>0.05).Antibiotic sensitivity test using standard disk diffusion technique was performed with 13 antibiotics. All theisolates were resistant (100%) to erythromycin, tetracycline, oxytetracyclin, enrofloxacin, ciprofloxacin,flumequin, lincospectin, furazolidon, and 100% of them were found to be susceptible to ceftriaxon andtiamulin, but 2 isolates (66.7%) were moderately sensitive to tylosin and amoxicillin and sensitive toflorfenicol. This study is the first report of the prevalence of ORT, bacterial isolation and molecular methodin broiler chickens after broiler breeder vaccination in Guilan province.
https://ijvr.shirazu.ac.ir/article_88_70e7d7cdd2c8e1494c908afedde641ff.pdf
2011-12-01
345
349
10.22099/ijvr.2011.88
Isolation
Identification
Ornithobacterium rhinotracheale
Broiler chickens
Y.
Asadpour
y_asadpour@yahoo.com
1
Department of Veterinary, Agricultural and Natural Resources Research Center, Guilan, Iran
LEAD_AUTHOR
M.
Banani
2
Department of Avian Disease Research and Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, Iran
AUTHOR
S. A.
Pourbakhsh
3
Department of Avian Disease Research and Diagnosis, Razi Vaccine and Serum Research Institute, Karaj, Iran
AUTHOR
ORIGINAL_ARTICLE
Study of the relationship between oxidative stress and subclinical mastitis in dairy cattle
Subclinical mastitis is considered as one of the most prevalent diseases in dairy cows, causing drasticloss in the dairy industry. Oxidative stress, induced by reactive oxygen species (ROS), is believed to be aprimary factor in various cattle diseases including mastitis, but there are few studies regarding the role ofROS in subclinical mastitis. This study was undertaken to i) study the changes in activities of erythrocyteglutathione peroxidase (e-GSH-Px) and its functional component, selenium (Se); ii) compare the activities of erythrocyte superoxide dismutase (e-SOD) and its functional components, Cu and Zn in cows withsubclinical mastitis and normal cows. Milk and heparinized blood samples were collected from 45 normalcows and 45 cows with subclinical mastitis from dairy cows in Tehran province, Iran. Somatic cell counts(SCC), activities of GSH-Px and SOD and concentrations of Cu, Zn and Se were measured. No significantdifference (P>0.05) was shown between GSH-Px and SOD activities and also between the concentrations ofCu and Zn in the studied groups. There was a marginal significant difference (P=0.05) between the Seconcentrations in the normal cows and cows with subclinical mastitis. The correlation between SCC andSOD was positive and significant (P<0.05). It was concluded that optimum antioxidant intake in the feedmay enhance the resistance against subclinical mastitis.
https://ijvr.shirazu.ac.ir/article_89_f92e790cdf3a6b96b3fabf8902104415.pdf
2011-12-01
350
353
10.22099/ijvr.2011.89
Subclinical mastitis
Oxidative stress
Glutathione peroxidase
Superoxide dismutase
Somatic cells
O.
Ghasemian Karyak
1
Graduated from Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
AUTHOR
S.
Safi
s.safi@srbiau.ac.ir
2
Department of Clinical Pathology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
LEAD_AUTHOR
A.
Rahimi Froushani
3
Department of Epidemiology and Biostatistics, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
AUTHOR
M.
Bolourchi
4
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Investigation of seroprevalance of toxoplasmosis in mares and stallions in Ankara province, Turkey
The aim of this study was to determine the seroprevalence of Toxoplasma gondii in mares and stallionsin the province of Ankara. Sera from 168 clinically healthy horses were tested for the presence ofToxoplasma gondii antibodies using the Sabin Feldman dye test. Toxoplasma gondii infection wasdetermined in 62 (36.9%) of 168 horses with the titers ranging from 1:16 to 1:256.
https://ijvr.shirazu.ac.ir/article_90_a3dcd1dbe6a0dbe37f224e84b4768849.pdf
2011-12-01
354
356
10.22099/ijvr.2011.90
Antibody
Mare
Sabin-Feldman dye test
Stallion
Toxoplasma gondii
S.
Gazyağci
gazyagciserkal@gmail.com
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, Kirikkale University, 71450 Yahsihan, Turkey
LEAD_AUTHOR
H. C.
Macun
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Kirikkale University, 71450 Yahsihan, Turkey
AUTHOR
C.
Babür
3
Department of Communicable Diseases Research, Refik Saydam National Hygiene Center, Ankara, Turkey
AUTHOR
ORIGINAL_ARTICLE
Surgical correction of a congenital partial ankyloglossia in a calf
A 40-day-old male Holstein breed calf with a complaint of inability of moving the tongue in and out aswell as difficulties with breastfeeding was referred to the veterinary clinic. A notch in the middle of thetongue tip was evident during oral examination. In addition, a thin tissue band between the ventral surface of the tongue and floor of the oral cavity was seen in the calf. A diagnosis of partial ventral ankyloglossia was made. Horizontal to vertical frenuloplasty was performed to correct the ankyloglossia. The day after thesurgery, greater mobility of the tongue was evident. It was concluded that most of the clinical signs,including problems during eating and breastfeeding were due to limitation of tongue movements. Horizontalto vertical frenuloplasty may improve clinical full function of tongue movement.
https://ijvr.shirazu.ac.ir/article_91_4f998041361c3c5e5afc2ee2bd745ae6.pdf
2011-12-01
357
359
10.22099/ijvr.2011.91
Calf
Ankyloglossia
Frenoluplasty
N.
Kiliç
nuh_kilic44@hotmail.com
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Adnan Menderes, 09016, PK 17, Aydin, Turkey
LEAD_AUTHOR