%0 Journal Article %T Identification of a cDNA sequence coding for kruppellike factor 2b (Klf2b) from the skin mucosa of common carp (Cyprinus carpio) %J Iranian Journal of Veterinary Research %I Shiraz University %Z 1728-1997 %A Jolodar, A. %A Seghatoleslami, S. %A Seyfiabad Shapouri, M. R. %A Mesbah, M. %D 2011 %\ 06/01/2011 %V 12 %N 2 %P 150-155 %! Identification of a cDNA sequence coding for kruppellike factor 2b (Klf2b) from the skin mucosa of common carp (Cyprinus carpio) %K Kruppel-like factor 2b (Klf2b) %K Common carp %R 10.22099/ijvr.2011.57 %X Kruppel-like factors (Klfs) are a highly related zinc-finger family of transcription factors implicated inthe regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. UsingRT-PCR technique, a 456 bp cDNA fragment encoding N-terminus part of a Klf2b was isolated from the skinmucosa of common carp (Cyprinus carpio) using two degenerative oligonucleotide primers. Use of thisfragment as a probe allowed the isolation of a larger cDNA clone through the searching of the GenBankexpressed sequence tag database. The size of the amplified product is 1157 bp, which encodes a polypeptide of 274 amino acid residues with a predicted molecular mass of 30.359 kDa and theoretical pI of 4.88. The deduced amino acid sequence exhibited 79, 54, and 53% identity to the homologous Klf2b identified from zebrafish Danio rerio, Spotted Green Pufferfish Tetraodon nigroviridis and Atlantic salmon Salmo salar, respectively. The common carp protein is 50% similar to Klf2 orthologues in African clawed frog Xenopus laevis, 44% in chicken Gallus gallus and is 30% similar to the mammalian Klf in house mouse Musmusculus. %U https://ijvr.shirazu.ac.ir/article_57_4243c5d7f2d6550b6bd71b56afcc2639.pdf