@article { author = {Norouzi, F. and Hashemitabar, Gh. R. and Razmi, G. R.}, title = {Detection of midgut antigens of Hyalomma anatolicum anatolicum tick using SDS-PAGE and Western blot}, journal = {Iranian Journal of Veterinary Research}, volume = {8}, number = {2}, pages = {166-169}, year = {2007}, publisher = {Shiraz University}, issn = {1728-1997}, eissn = {2252-0589}, doi = {10.22099/ijvr.2007.2690}, abstract = {Ticks are important ectoparasites which are a considerable threat to human beings and to animals all overthe world. Enormous economic losses annually occur in livestock production around the world as a result oftheir existance. One of the ways to control ticks and tick-borne diseases is to introduce resistance to theseectoparasites through immunization. For identification of the putative protective antigens, screening of largenumber of parasite antigens and their fractions are necessary. In this study, midguts of fed adult femaleHyalomma anatolicum anatolicum were used to prepare antigen and to identify the midgut profile.Polypeptide profile was analysed by SDS-PAGE with 12.5% concentration under denaturated conditions anddiscontinuous buffer system. Humoral immunity and antigenic pattern were evaluated by Western blot. Atotal of 4 fractions were observed in the polypeptide profile. The molecular weight of the fractions were 97,84, 66 and 55 kDa. The band with molecular weight of 66 kDa was dominant. Positive reaction with 84, 66and 55 kDa bands were observed in immuno-blot of the midgut antigens.}, keywords = {Hyalomma anatolicum anatolicum,Midgut antigens,SDS-PAGE,Western blotting}, url = {https://ijvr.shirazu.ac.ir/article_2690.html}, eprint = {https://ijvr.shirazu.ac.ir/article_2690_08ff50ca2278ad8c99419ca72be2bb3d.pdf} }