Screening the partial coding region of metallothionein isoform-2 gene in Zebu cattle
P. N.
Gholap
MVSc, Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Seminary Hills, Nagpur-440006, Maharashtra State, India
author
D. S.
Kale
Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Seminary Hills, Nagpur-440006,
Maharashtra State, India
author
K.
Krishnamurthi
3Environmental Health Division, National Environmental Engineering Research Institute (NEERI), Nehru
Marg, Nagpur-440020, Maharashtra State, India
author
A. R.
Sirothia
Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Seminary Hills, Nagpur-440006,
Maharashtra State, India
author
M. D.
Kothekar
Department of Animal Genetics and Breeding, Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences University (MAFSU), Seminary Hills, Nagpur-440006,
Maharashtra State, India
author
text
article
2016
eng
Metallothionein (MT) is important because it binds tightly to heavy metals to decrease their toxicity. DNA was isolated from 30 toxic metal exposed and 30 toxic metal unexposed Zebu cows. The amplified metallothionein isoform-2 (MT-2) PCR product (489 bp) was further used for PCR-RFLP and DNA sequencing. MT-2 TaqI PCR-RFLP revealed homozygous genotype (AA) except for the E23 animal (AB). The genotype frequency of AA and AB (E23) genotypes in the exposed groups was 0.967 and 0.033 respectively. DNA sequencing was carried out for the toxic metal exposed sample (E23) and the control group sample (C13). Blast comparisons of the sequences were then aligned against a nucleotide database which revealed 150 nucleotide substitutions consisting of 70 transitions and around 80 transversions. DNA sequencing followed by PCR-RFLP for MT-2 revealed a higher number of nucleotide substitutions (150) for the AB genotype of E23 as compared to the AA genotype (38) of E21. The proportions of transversion mutations in the AB genotype were higher as compared to the MT-2 AA genotype. DNA sequencing was carried out based on random sampling for E21 and C13. Alignment analysis of the E21 and C13 sample revealed 38 nucleotide substitutions consisting of equal numbers of transition and transversion. BLAST analysis of the identified partial sequence revealed 89% identity with Bos taurus, 85% identity with sheep, 98% identity with buffalos and 100% identity with goat MT-2 sequences. Overall findings of the present study revealed DNA sequence variation in the coding region of the MT-2 gene of Zebu cattle which can be utilised to characterize and explore markers for heavy metal homeostasis in Zebu cattle.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
155
159
https://ijvr.shirazu.ac.ir/article_3807_e5962d2722840baa5476cb0688c113ae.pdf
dx.doi.org/10.22099/ijvr.2016.3807
Genomic diversity of Clostridium perfringens strains isolated from food and human sources
A.
Afshari
Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
author
A.
Jamshidi
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
J.
Razmyar
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
M.
Rad
Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2016
eng
Clostridium perfringens is a serious pathogen which causes enteric diseases in domestic animals and food poisoning in humans. Spores can survive cooking processes and play an important role in the possible onset of disease. In this study, RAPD-PCR and REPPCR were used to examine the genetic diversity of 49 isolates of C. perfringens type A from three different sources. The results of RAPD-PCR revealed the most genetic diversity among poultry isolates, while human isolates showed the least genetic diversity. Cluster analysis obtained from RAPD-PCR and based on the genetic distances split the 49 strains into five distinct major clusters (A, B, C, D, and E). Cluster A and C were composed of isolates from poultry meat, cluster B was composed of isolates from human stool, cluster D was composed of isolates from minced meat, poultry meat and human stool and cluster E was composed of isolates from minced meat. Further characterization of these strains by using (GTG) 5 fingerprint repetitive sequence-based PCR analysis did not show further differentiation between various types of strains. In conclusion, RAPD-PCR method seems to be very promising for contamination source tracking in the field of food hygiene.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
160
164
https://ijvr.shirazu.ac.ir/article_3809_6b1f59cabf772af8c53754a30653d2a7.pdf
dx.doi.org/10.22099/ijvr.2016.3809
Effects of cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes
K. Gh. M.
Mahmoud
Department of Animal Reproduction & Artificial Insemination (AI), National Research Center, Dokki, Giza, Egypt
author
M. M. M.
El-Sokary
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
author
M. M. M.
Kandiel
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
author
M. E. A.
Abou El-Roos
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
author
G. M. S.
Sosa
Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
author
text
article
2016
eng
The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium with no cysteamine (control) or 50 μM cysteamine (treated). Oocytes were vitrified in vitrification solution 1 (VS1): 1.5 M ethylene glycol (EG) + 1.5 M dimethyl sulfoxide (DMSO) for 45 s (step one). After this initial exposure, oocytes were transferred to VS2: 3 M EG + 3 M DMSO in a holding medium for 25 s (step two). After warming, oocytes were evaluated morphologically and then cultured for a further 2 h in the cysteamine-supplemented or control maturation media. The oocytes were evaluated morphologically, stained with trypan blue for viability evaluation. The maturation rate of oocytes was higher (P<0.05) for IVM media with cysteamine compared with controls. There was no significant difference in morphology, survivability and maturation rate between the twovitrification groups (cysteamine-treated and untreated groups) but the morphology, survivability and percentages of metaphase-II oocytes in both groups of vitrified oocytes were lower compared with their respective controls. In conclusion, the addition of cysteamine to the maturation medium improved nuclear maturation of buffalo oocytes but had no positive effect on their cryoresistance during vitrification.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
165
170
https://ijvr.shirazu.ac.ir/article_3810_84f13048b69ef0e460382d50217673e1.pdf
dx.doi.org/10.22099/ijvr.2016.3810
Evaluating a murine model of endometritis using uterine isolates of Escherichia coli from postpartum buffalo
S. H.
Dar
Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
S.
Qureshi
Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
M.
Palanivelu
Divsion of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
S.
Muthu
Division of Temperate Animal
Husbandry, Indian Veterinary Research Institute, Mukteswar, Uttarakhand, 263138, India
author
S.
Mehrotra
Division of Livestock Production and
Management, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
M. H.
Jan
Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
G. R.
Chaudhary
Ph.D. Scholar, Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
H.
Kumar
Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
R.
Saravanan
Immunology Section, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
K.
Narayanan
Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243122, India
author
text
article
2016
eng
Ascending infection of the uterus with Gram-negative bacteria is responsible for postpartum endometritis in cattle and buffalo and can adversely affect fertility. Development of a laboratory animal model for bovine endometritis would facilitate the understanding of the pathogenesis as it is difficult to conduct controlled experimentation in the native host. In the present study, 30 virgin Swiss Albino mice (5-8 weeks old) were used to evaluate the pathogenic potential of Escherichia coli, isolated from the normally calved postpartum buffalo to induce endometritis. Mice in the diestrus phase of the estrous cycle were randomly allotted toone of the following four intravaginal inoculation (100 μL) treatments: EG (experimental group)-1: sterile normal saline; EG-2, -3 and -4: E. coli@ 1.5 × 104, 105 and 106 CFU/ml, respectively. The animals were then scarified 36 h post-inoculation to study gross and microscopical lesions. Gross changes were confined to EG-4. Acute endometritis was recorded in 50% of the EG-3 and 66.7% of the EG-4. The rate of acute endometritis development was significantly higher in EG-4 (P<0.05) as compared to the other groups. The present study demonstrated that the animal model for bubaline endometritis can be developed in mice by intravaginal inoculation of E. coli@ 1.5 × 106 CFU/ml at diestrus. Ease of intravaginal inoculation, apparent absence of systemic involvement and high infective rate are the advantages of the model over other studies.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
171
176
https://ijvr.shirazu.ac.ir/article_3811_034081d92eac940d62cd6fe2eb98bedf.pdf
dx.doi.org/10.22099/ijvr.2016.3811
PFGE genotyping and molecular characterization of Campylobacter spp. isolated from chicken meat
B.
Bakhshi
Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
author
M.
Kalantar
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
A.
Rastegar-Lari
Antimicrobial Resistance Research
Center, Iran University of Medical Sciences, Tehran, Iran
author
F.
Fallah
Pediatric Infection Research Center, Shahid Beheshti University of
Medical Sciences, Tehran, Iran
author
text
article
2016
eng
A total of 70 samples were collected from chicken meat obtained from 10 markets in Tehran, Iran from which 39 Campylobacter coli were isolated. Among 10 antibiotics used, maximum resistance was seen to trimethoprim-sulphamethoxazole (SXT) (97.36%), nalidixic acid (94.8%), ciprofloxacin (87.7%), streptomycin (89.72%), and tetracycline (97.4%). No resistance was to gentamycin was observed. None of the Campylobacter strains under study harbored integron, suggesting the involvement of other resistancemechanisms in emergence of multi drug resistance (MDR) phenotype among the isolates. Two major types (A and B) and 15 subtypes (A1-A8 and B1-B7) were identified. Pulsed-field gel electrophoresis (PFGE) analysis demonstrated a high degree of homogeneity while the majority of the isolates shared identical or very similar PFGE genotypes. Isolates with identical genotypes differed in their resistance profile, although all of them assigned to MDR phenotype. To our knowledge, this is the first molecular survey from Iran characterizing Campylobacter isolates from poultry, which adds to our knowledge the epidemiological linkage ofCampylobacter isolates with MDR properties from different sources and emphasizes the need for cautious use of antimicrobials in different fields of food production chain.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
177
183
https://ijvr.shirazu.ac.ir/article_3812_c8a278de484849c18211c55b15bbb5f8.pdf
dx.doi.org/10.22099/ijvr.2016.3812
Proteomic analysis of muscle tissue from rainbow trout (Oncorhynchus mykiss) fed dietary β-glucan
Gh.
Ghaedi
Graduated from Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr,
Iran
author
S.
Keyvanshokooh
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology,
Khorramshahr, Iran
author
H.
Mohammadi Azarm
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology,
Khorramshahr, Iran
author
M.
Akhlaghi
Department of Aquatic Animal Health and Diseases, School of Veterinary Medicine, Shiraz University, Shiraz,
Iran
author
text
article
2016
eng
The aim of this study was to examine the changes in muscle proteome of the rainbow trout fed dietary β-glucan. The experimental diets contained 0 (control), 0.1% and 0.2% β-1,3/1,6 yeast glucan. First, feeding larvae were fed to apparent satiation nine times per day with their respective diets over two months. The percentage of body weight gain and feed efficiency of fish fed 0.2% diet was significantly higher than those of fish fed the control and 0.1% diets. Fish fed the control and 0.2% diets were subjected to proteomic analysis. Proteins of the muscle tissue were analyzed using two-dimensional electrophoresis and mass spectrometry. Spots that were found to differ significantly in abundance between control and β-glucan fed fish were selected for identification. Out of 8 protein spots showing differential expression, 7 spots were successfully identified. Two protein spots that were found to be increased in abundance in the β-glucan treated rainbow trout corresponded to tropomyosin alpha-1 chain (spot 1) and slow myotomal muscle tropomyosin (spot 2). The five spots that were down-regulated with dietary β-glucan supplementation were identified as different forms of myosin: myosin light polypeptide 3-2 (spot 3), myosin light chain 1 (spots 4 and 5), fast myosin light chain 2 (spot 6) and myosin heavy chain (spot 7). The altered expression of structural proteins in fish fed β-glucan may be related to higher growth rate in rainbow trout. These findings provide basic information to understand possible mechanisms of dietary β-glucan contribution to better growth in rainbow trout.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
184
189
https://ijvr.shirazu.ac.ir/article_3813_8043ca01e4556fe56cd4e7aecbe54423.pdf
dx.doi.org/10.22099/ijvr.2016.3813
The influence of administration of different doses of arginine and lysine coupled with zinc in the lactating ewes on the zinc concentration in milk
M.
Keshvari
MSc in Animal Physiology, Department of Physiology, Faculty of Biological Science, Shahid Beheshti University, Tehran, Iran
author
H.
Khazali
Department of Physiology, Faculty of Biological Science, Shahid Beheshti University, Tehran, Iran
author
H.
Rokni
Department of Education,
Applied Scientific Education Institute of Agriculture-Jahad, Tehran, Iran
author
A.
Hosseini
Ph.D. Student in Animal Physiology, Department of Physiology, Faculty of Biological Science, Shahid Beheshti University, Tehran, Iran
author
text
article
2016
eng
Zinc (Zn) plays an essential role in the human and animal body system. Zinc deficiency might cause many disorders, so it is important to provide a sufficient amount of this microelement in both animals and humans. One of the most important sources of Zn is milk. The purpose of this study was to determine whether Zn can pass through the mammary epithelial cell into milk by a cotransport system with amino acid in the lactating ewes. This experimental research included 54 lactating ewes collected from the Agriculture-Jahad Organization of Tehran Province, that were allocated into 9 groups as follows: groups which received inorganic form as Zn chloride (1, 2 and 4 mg/kg), and groups which received organic Zn with different doses of arginine or lysine (1, 2 and 4 mg/kg) in chelate form. Milk samples were taken 30 min before and 6 h after intravenous injection of Zn preparations. Zinc concentration in milk was measured using the flame atomic absorption spectrometric method. There were no significant differences (P>0.05) between groups in Zn concentration of the milk before administration of inorganic or organic Zn. Data analysis showed that administration of inorganic Zn caused a significant increase of Zn concentration in the milk in a dose dependent manner. While administration of arginine or lysine with Zn in chelate form caused a significant decline in Zn concentration of milk compared toinorganic Zn in a dose dependent manner. According to our results, administration of different forms of Zn influenced the Zn concentration in milk.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
190
193
https://ijvr.shirazu.ac.ir/article_3814_ab3715632849435cc0ad4615b8b6b7cb.pdf
dx.doi.org/10.22099/ijvr.2016.3814
Mitochondrial DNA sequence-based phylogenetic relationship of Trichiurus lepturus (Perciformes: Trichiuridae) from the Persian Gulf
S.
Tamadoni Jahromi
Department of Genetic, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran
author
S. A.
Mohd Noor
Department of Biology, School of
Biological Sciences, University Science Malaysia (USM), Penang, Malaysia
author
K.
Pirian
Department of Biotechnology, School of Agricultural Sciences, Bu-Alisina University, Hamadan, Iran
author
R.
Dehghani
Department of Biology, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar
Abass, Iran
author
M.
Nazemi
Department of Genetic, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abass, Iran
author
A.
Khazaali
Young Researchers and Elite Club, Bandar Abass Branch, Islamic Azad University, Bandar Abass, Iran
author
text
article
2016
eng
In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship of Trichiurus lepturus in the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs to T. lepturus showing 42 divergence sites among the same reported partial sequences of 16S rRNA gene from the other area (West Atlantic and Indo-Pacific area). Phylogeny results showed that all 18 haplotypes of the species clustered into five clades with reasonably high bootstrap support of values (>64%). Overall, the tree topology for both phylogenetic and phenetic trees for 16S rDNA was similar. Both trees exposed two major clusters, one wholly containing the haplotypes of the T. lepturus species belonging to Indo-Pacific area with two major sister groups including Persian Gulf specimen and the other cleared the Western Atlantic and Japan individuals clustered in another distinct clade supporting the differentiation between the two areas. Phylogenic relationship observed between the Persian Gulf and the other Indo-Pacific Individuals suggested homogeneity between two mentioned areas.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
194
199
https://ijvr.shirazu.ac.ir/article_3815_5394e2594c4a097a802a6764f268139f.pdf
dx.doi.org/10.22099/ijvr.2016.3815
Immunostimulatory effect of the aqueous leaf extract of Phyllanthus niruri on the specific and nonspecific immune responses of Oreochromis mossambicus Peters
M.
Muthulakshmi
MSc in Zoology, Science Section, Government Higher Secondary School, Avadi, Chennai-600062, India
author
P. A.
Subramani
Ph.D. Student in Nanobiotechnology, Centre for Fish Immunology, School of Life Sciences, Vels University, Pallavaram, Chennai-600117, India
author
R. D.
Michael
Centre for Fish Immunology, School of Life Sciences, Vels University, Pallavaram, Chennai-600117, India
author
text
article
2016
eng
Plant derived immunostimulants are a promising alternative to chemotherapeutics and also perhaps vaccines. In the present study, we examined the immunostimulating properties of aqueous leaf extract of Phyllanthus niruri, an Indian traditional medicinal herb, on neutrophil activation and antibody response of Oreochromis mossambicus (Peters). Serial ten-fold diluted doses of P. niruri ranging from 0.002 mg to 20 mg were administered to two groups of O. mossambicus (n=8). One group of fishes was administered with sheep red blood cells and the primary and secondary antibody responses were estimated using direct haemagglutination assay. The other group of fishes was administered heat-aggregated BSA to assess the ability of plant extract to elicit neutrophil activation. Our results indicate a significant enhancement of both neutrophil activation and antibody response. Among the various doses tested, fishes administered 20 mg/kg body weight caused the maximal enhancement of both primary and secondary antibody response and 0.002 mg/kg showed higher neutrophil activation compared to that of the control group. This short study indicates that aqueous leafextract of P. niruri has the potential to be used as an immunostimulant and after confirming its immunostimulatory properties by a battery of tests on other nonspecific and specific parameters and disease-protective property by challenging the fish with virulent fish pathogens, it can be used either as a routine feed supplement to activate the immune system of farmed fishes or as an adjuvant to enhance the efficacy of vaccines.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
200
202
https://ijvr.shirazu.ac.ir/article_3817_130a7d6bbbcc4b215b9a59b56c86d6c1.pdf
dx.doi.org/10.22099/ijvr.2016.3817
Effects of hydro-alcoholic extract of Vitex agnus-castus fruit on kidney of D-galactose-induced aging model in female mice
A. A.
Oroojan
Ph.D. Student in Physiology, Department of Physiology, Student Research Committee, School of Medicine, Ahvaz Jundishapur
University of Medical Science, Ahvaz, Iran
author
A.
Ahangarpour
Health Research Institute, Diabetes Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
L.
Khorsandi
Cell & Molecular Research Center, Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
S. A.
Najimi
MSc in Physiology, Physiology Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
author
text
article
2016
eng
The aim of the present study was to evaluate the effect of a hydro-alcoholic extract of Vitex agnus-castus (VAC) fruit on blood urea nitrogen (BUN), creatinine (Cr) and, kidney histology of a female mouse model of D-galactose induced aging. In this experimental study, 72 NMRI mice were divided into 6 groups: control, VAC, D-galactose, D-galactose+VAC, aging, and aging+VAC. D-galactose was injected for 45 days and, VAC extract administered in the last 7 days, twice a day. Serum BUN and Cr levels were not significantly changed in the D-galactose and natural aged animals in comparison to control group. Histological changes such as nuclear pyknosis, proximal cell swelling, infiltration of inflammatory cells, tubular dilatation and, vasodilatationwere observed in both D-galactose and natural aged mice. Further, glomerules diameter was decreased in them. Administration of VAC could attenuate the histological alterations. These results indicate that VAC may have beneficial effects on aging and aging related kidney disease.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
203
206
https://ijvr.shirazu.ac.ir/article_3818_5a33f3ae3d7b22f2f41f9dde06f7f32b.pdf
dx.doi.org/10.22099/ijvr.2016.3818
Estrogen receptor gene 1 expression in caprine and its effect on fertility
S.
Saraswat
Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
author
P. K.
Rout
GGB Division, Central Institute for Research on goats (CIRG), Makhdoom, Farah- 281122, Mathura, Uttar Pradesh, India
author
S. D.
Kharche
Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
author
A. K.
Goel
Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
author
S. K.
Jindal
Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
author
S.
Kumar
Physiology Reproduction and Shelter Management (PRSM) Division, Central Institute for Research on Goats (CIRG), Makhdoom, Farah-281122, Mathura, Uttar Pradesh, India
author
text
article
2016
eng
The present study was undertaken to analyze the expression pattern of estrogen receptor 1 gene (ESR1) in Barbari bucks (fertile and non-fertile) identified on the basis of seminal quality traits and fertility trials. RNA was extracted from the spleen by Trizol method. The expression pattern of ESR1 gene was analyzed using real time polymerase chain reaction (RT-PCR). The expression pattern of ESR1 gene was analyzed by RT-PCR (Roche LC-480). Relative quantification by RT-PCR indicated that the ESR1 gene expression showed more fold in fertile bucks as compared to non-fertile.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
207
209
https://ijvr.shirazu.ac.ir/article_3819_7d9981df9f16c3b82cdcdbd1219f0e4e.pdf
dx.doi.org/10.22099/ijvr.2016.3819
Relationship between white spot syndrome virus (WSSV) loads and characterizations of water quality in Litopenaeus vannamei culture ponds during the tropical storm
J. S.
Zhang
Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
Z. J.
Li
Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
G. L.
Wen
Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
Y. L.
Wang
MSc in Aquaculture, Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
L.
Luo
Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries
Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
H. J.
Zhang
MSc in Aquaculture, Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
H. B.
Dong
Key Laboratory South China Sea Fisheries Resources Exploitation & Utilization, Ministry of Agriculture, South China Sea Fisheries
Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510000, China
author
text
article
2016
eng
An in-situ experiment was conducted to investigate the effect of tropical storm on the white spot syndrome virus (WSSV) loads in Litopenaeus vannamei rearing ponds. White spot syndrome virus loads, heterotrophic bacteria, Vibrio and water quality (including temperature, dissolved oxygen (DO), salinity, pH, NH4-N, and NO2-N) were continually monitored through one tropical storm. The WSSV loads decreased when tropical storm made landfall, and substantially increased when typhoon passed. The variation of WSSV loads was correlated with DO, temperature, heterotrophic bacteria count, and ammonia-N concentrations. These results suggestedthat maintaining high level DO and promoting heterotrophic bacteria growth in the shrimp ponds might prevent the diseases’ outbreak after the landfall of tropical storm.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
3
no.
2016
210
214
https://ijvr.shirazu.ac.ir/article_3820_c4a38baf6694273c4e0df6e590290096.pdf
dx.doi.org/10.22099/ijvr.2016.3820