Comparative efficacy of Prolene and Prolene-Vicryl composite mesh for experimental ventral hernia repair in dogs
H.
Anjum
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
S. G.
Bokhari
Pet Centre, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M. A.
Khan
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M.
Awais
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
Z. U.
Mughal
Ph.D. Scholar, Pet Centre, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
H. K.
Shahzad
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
F.
Ijaz
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M. I.
Siddiqui
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
I. U.
Khan
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
A. S.
Chaudhry
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
R.
Akhtar
Department of Pathology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
S.
Aslam
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
H.
Akbar
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M.
Asif
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M. K.
Maan
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M.A.
Khan
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
A.
Noor
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
W. A.
Khan
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
A.
Ullah
Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
M. A.
Hayat
Ph.D. Scholar, Department of Clinical Medicine & Surgery, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan
author
text
article
2016
eng
In this study, efficacy of two hernia mesh implants viz. conventional Prolene and a novel Prolene-Vicryl composite mesh was assessed for experimental ventral hernia repair in dogs. Twelve healthy mongrel dogs were selected and randomly divided into three groups, A, Band C (n=4). In all groups, an experimental laparotomy was performed; thereafter, the posterior rectus sheath and peritoneum were sutured together, while, a 5 × 5 cm defect was created in the rectus muscle belly and anterior rectus sheath. For sublay hernioplasty, the hernia mesh (Prolene: group A; Prolene-Vicryl composite mesh: group B), was implanted over the posterior rectus sheath. In group C (control), mesh was not implanted; instead the laparotomy incision was closed after a herniorrhaphy. Post-operative pain, mesh shrinkage and adhesion formation were assessed as short term complications. Post-operatively, pain at surgical site was significantly less (P<0.001) in group B (composite mesh); mesh shrinkage was also significantly less in group B (21.42%, P<0.05) than in group A (Prolene mesh shrinkage: 58.18%). Group B (composite mesh) also depicted less than 25% adhesions (Mean ± SE: 0.75 ± 0.50 scores, P≤0.013) when assessed on the basis of a Quantitative Modified Diamond scale; a Qualitative Adhesion Tenacity scale also depicted either no adhesions (n=2), or, only flimsy adhesions (n=2) in group B (composite mesh), in contrast to group A (Prolene), which manifested greater adhesion formation and presence of dense adhesions requiring blunt dissection. Conclusively, the Prolene-Vicryl composite mesh proved superior to the Prolene mesh regarding lesser mesh contraction, fewer adhesions and no short-term follow-up complications.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
78
83
https://ijvr.shirazu.ac.ir/article_3730_a4bc1cb45d57776899639e4c404d1b4e.pdf
dx.doi.org/10.22099/ijvr.2016.3730
Evaluation of immunogenicity and protective efficacy of recombinant ptfA of avian Pasteurella multocida
Q.
Gong
Department of Biotechnology, College of Food and Bioengineering, Henan University of Science and Technology, Henan Engineering Laboratory of Livestock Disease Diagnosing and Food Safety Testing, Luoyang, 471023, China
author
N.
Qu
Department of Biotechnology, College of Food and Bioengineering, Henan University of Science and Technology, Henan Engineering Laboratory of Livestock Disease Diagnosing and Food Safety Testing, Luoyang, 471023, China
author
M. F.
Niu
Department of Biotechnology, College of Food and Bioengineering, Henan University of Science and Technology, Henan Engineering Laboratory of Livestock Disease Diagnosing and Food Safety Testing, Luoyang, 471023, China
author
C. L.
Qin
Department of Biotechnology, College of Food and Bioengineering, Henan University of Science and Technology, Henan Engineering Laboratory of Livestock Disease Diagnosing and Food Safety Testing, Luoyang, 471023, China
author
text
article
2016
eng
Avian Pasteurella multocida is the causative agent of fowl cholera, a disease much affecting the poultry industry. In order to study the efficacy of the recombinant subunit vaccine constructed with ptfA gene of avian P. multocida, the ptfA gene fragment amplified by PCR from avian P. multocida was cloned into the prokaryotic expression vector pET32a and the recombinant plasmid pET32a-ptfA was obtained. The pET32a-ptfA was expressed in Escherichia coli BL21(DE3) and the target protein rPtfA was purified. The purified protein was then mixed with Freund’s adjuvant and the recombinant subunit vaccine was obtained. Three groups of chickens labeled as rPtfA, attenuated live vaccine and PBS were vaccinated with the recombinant subunit vaccine, attenuated live vaccine and PBS, respectively. Serum antibodies, peripheral blood lymphocyte proliferation (PBLP) and interferon-γ (IFN-γ) level secreted by peripheral blood lymphocyte were tested. The immunized chickens were finally challenged with virulent avian P. multocida and the protection rate was counted. Indirect ELISA showed the levels of antibodies in rPtfA and attenuated vaccine groups were most significantly higher than the other groups (P<0.01), and the former was slightly lower than the latter. Peripheral blood lymphocyte proliferation experiments and IFN-γ experiments indicated that SI value and the levels of IFN-γ induced by ConA in the two vaccine groups were significantly higher than those of the PBS groups (P<0.01), and that the attenuated vaccine group was higher than the rPtfA group. The protection rates of rPtfA and attenuated live vaccines were 45% and 75%, respectively. The results indicated that the PtfA recombinant subunit vaccine was capable of improving the immunity level and inducing a protective effect for the vaccinated chickens, but it was barely satisfactory.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
84
88
https://ijvr.shirazu.ac.ir/article_3731_69ba41b6416a58c87c94cd70f5376501.pdf
dx.doi.org/10.22099/ijvr.2016.3731
Molecular characterization and phylogenetic analysis of bovine viral diarrhea virus in dairy herds of Fars province, Iran
A.
Khodakaram-Tafti
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
A.
Mohammadi
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
GH.
Farjani Kish
Ph.D. Student in Veterinary Pathology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran, and Department of Pathobiology, School of Veterinary Medicine, Lorestan University, Khorramabad, Iran (Present address)
author
text
article
2016
eng
Bovine viral diarrhea virus (BVDV) is one of the most important viral pathogens of cattle worldwide. The aim of present study was to determine the molecular characterization and phylogenetic analysis of BVDV infection in dairy herds of Fars province, Iran. For initial screening, a total of 400 blood samples were collected from 12 industrial dairy herds with previous history of diarrhea, abortion or birth of weak calves and analyzed using reverse transcription-polymerase chain reaction (RT-PCR) on buffy coat. In the next step, blood samples and also ear notch biopsies were collected from 100 cattle of infected farms three weeks later which were subsequently tested by antigen capture ELISA (ACE), RT-PCR and immunohistochemistry (IHC). The results of nested RT-PCR were successful in 16 out of 400 buffy coat samples (4%) in the initial screening. Also, 8 out of 100 samples (8%) were positive by all practiced tests including RT-PCR, ACE and IHC on buffy coat, serum and skin samples, respectively. Immunoreactivity for bovine BVDV antigen as brown, coarsely to finely granular was observed within the cytoplasm of epidermic epithelial cells, hair follicles and subcutaneous stromal cells. Genetic sequence analyses showed both genotypes, BVDV-1 and BVDV-2. The new isolates were identified as BVDV1-FarsA, BVDV1-FarsB and BVDV2-FarsA in the phylogenetic tree. Since both genotypes of the virus are present in the region, our findings emphasize the importance of monitoring BVDV infection in cattle and suggest detection and elimination of PI animals for controlling and eradication of BVDV in Fars province.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
89
97
https://ijvr.shirazu.ac.ir/article_3732_a3b1f7989a93f7756675a3d6b292f006.pdf
dx.doi.org/10.22099/ijvr.2016.3732
Prevention and treatment of Encephalitozoon cuniculi infection in immunosuppressed rabbits with fenbendazole
S. S.
Abu-Akkada
Department of Parasitology, Faculty of Veterinary Medicine, Alexandria University, Edfina, Behera, Egypt
author
S. S.
Oda
Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Edfina, Behera, Egypt
author
text
article
2016
eng
This study was conducted to evaluate the efficacy of oral administration of fenbendazole (20 mg/kg body weight) prior to and after experimental infection of immunosuppressed rabbits with Encephalitozoon cuniculi. A total of thirty rabbits were divided into five groups: NN (non-immunosuppressed; non-infected), IN (immunosuppressed; non-infected), IPI (immunosuppressed; protected-infected), ITI (immunosuppressed; treated-infected), and II (immunosuppressed; infected) groups. Fenbendazole was administered as a prophylactic for seven successive days before infection with E. cuniculi and as a treatment for four weeks initiated on the 28th day post-challenge (PC). Experimental rabbits were infected with intraperitoneal injection of 2 × 105 E. cuniculi spores. Parameters evaluated were body weight, detection of spores in urine, serum antibody assay, hematological, biochemical and histopathological changes. The IPI and ITI groups showed a significant better final bwt than the II group. Spores were detected in urine of all infected rabbits from the 28th day PC until the end of the study. The IPI group showed the least values of antibodies (IgG) compared to the ITI and II groups. Concerning histopathological changes, the intensity of the lesions was marked particularly in the II rabbits and to a lesser extent in the ITI rabbits. Noticeable improvement was found in the IPI rabbits. It could be concluded that fenbendazole was effective to some extent in protection of rabbits against E. cuniculi infection, while when administered as a therapeutic no significant effects were observed.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
98
105
https://ijvr.shirazu.ac.ir/article_3733_e95e0e54a435c6bc68fdb3e8298be38e.pdf
dx.doi.org/10.22099/ijvr.2016.3733
Development of anti-Helicobacter pylori immunoglobulins Y (IgYs) in quail
S.
Najdi
Ph.D. Student in Microbiology, Department of Pathobiology Science, College of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
G.
Nikbakht Brujeni
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
N.
Sheikhi
Department of Clinical Science, College of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
S.
Chakhkar
Department of Clinical Science, College of Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
text
article
2016
eng
Helicobacter pylori (H. pylori) is a gram-negative, microaerophilic bacterium that cause the stomach infection in more than 50% of human population worldwide. The aim of this study was to examine the possibility of anti-H. pylori immunoglobulins Y (IgYs) production in quails and evaluate the effect of the different methods of immunization on titers of IgY in egg yolks. Whole cell bacterial antigen was used for immunization of quails. Forty Japanese quails (Coturnix japonica) were divided into four groups. The first group intramuscularly immunized with one dose of antigen (3 × 108 inactivated bacteria) whereas the second group injected with half dose. Third group administered orally. Yolk IgY was isolated using precipitation method of water dilution combined with chloroform. Dot-blot and ELISA (enzyme-linked immunosorbent assay) were used for determining the specificity and quantifying the titer of IgY in egg yolks. Results showed that quails as well as chickens are able to produce anti-H. pylori IgY. Quails antibodies have high titer and specificity that can be used in therapeutic and research purposes. This study indicated that higher amounts of antigen can not develop higher titer of IgY and injection is not necessary for efficient immunization of the quail against H. pylori.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
106
110
https://ijvr.shirazu.ac.ir/article_3734_e8f19e0dcf19143a9cd83d8c0ad68044.pdf
dx.doi.org/10.22099/ijvr.2016.3734
Effect of substituting increasing levels of organic Zn for inorganic Zn on performance, hematological and serum biochemical constituents, antioxidant status and immune response in rat
D.
Nagalakshmi
Department of Animal Nutrition, College of Veterinary Science, PVNR Telangana Veterinary University, Hyderabad-50030, Telengana, India
author
K.
Sridhar
Ph.D. Scholar in Animal Nutrition, Department of Animal Nutrition, College of Veterinary Science, PVNR Telangana Veterinary University, Hyderabad-50030, Telengana, India
author
P. S.
Swain
Ph.D. Scholar in Animal Nutrition, Department of Animal Nutrition, National Dairy Research Institute, Karnal, Haryana, India
author
A. G.
Reddy
Department of Pharmacology and Toxicology, College of Veterinary Science, PVNR Telangana Veterinary University, Hyderabad-50030, Telengana, India
author
text
article
2016
eng
The effect of replacing dietary Zn supplemented from inorganic (ZnCO3) source with organic Zn (Zn methionine; Zn-met) was investigated in 72 rats (98.42 ± 1.483 g) by randomly allotting to 4 diets (6 replicates/diet, 3 rats/replicate). Basal diet was prepared with purified ingredients without Zn. The control diet (AIN-76A) contained 12 ppm of Zn from ZnCO3 (100-I). In the other diets ZnCO3 was replaced with Zn-met at the rates of 50 (50I:50O), 75 (25I:75O) or 100% (100-O). Weekly body weight and daily feed intake were recorded for 14 weeks. Blood was collected by retro-orbital puncture on the 70th and 80th day to determine haematological and various serum biochemical constituents, and antioxidant enzyme activities in haemolysate, respectively. Rats were antigenically challenged with sheep RBC on day 73 to assess humoral immune response (HIR), and on day 95 for cell mediated immune response (CMIR) and rats were sacrificed at the end of rearing period to collect liver, muscle, pancreas and kidneys for Zn estimation and oxidative stress markers in liver. The data were analysed using completely randomized design. Weight gain and feed intake, hematological and serum biochemical constituents, Zn content in organs (except liver) were not influenced by replacing ZnCO3 with Zn-met. Zinc concentrations in the serum and liver were higher (P<0.05) with 50% replacement of ZnCO3 with Zn-met compared to 0 or 100% replacement. Lower (P<0.05) lipid peroxidation and higher (P<0.05) glutathione peroxidase and glutathione reductase activities were observed with 50 and 75% replacement of ZnCO3 with Zn-met compared to 0 or 100% replacement. Protein carbonyls and reduced glutathione in liver were not affected, while TBARS decreased (P<0.05) with substituting Zn-met (50-100%) for ZnCO3. The HIR and CMIR increased with increasing Zn-met supplementation and the highest response was observed with 75-100% replacement of ZnCO3 with Zn-met. It is concluded that replacement of 50 or 75% of ZnCO3 with Zn-met increased antioxidant and immune response in rats with no effect on growth.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
111
117
https://ijvr.shirazu.ac.ir/article_3735_8fb822339b934700b8d5fcbe4ac1e7a7.pdf
dx.doi.org/10.22099/ijvr.2016.3735
The superiority of paracostal endoscopic-assisted gastropexy over open incisional and belt loop gastropexy in dogs: a comparison of three prophylactic techniques
A.
Tavakoli
Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
author
M.
Mahmoodifard
Department of Clinical Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran;Resident of Veterinary Surgery, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
A. H.
Razavifard
Graduated from Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
author
text
article
2016
eng
Prophylactic gastropexy is a procedure that prevents the occurrence of a life threatening condition known as gastric dilation and volvulus (GDV) in dogs. The objective of this study was to compare incisional, belt loop and minimally invasive endoscopically assisted gastropexy by evaluating different parameters such as surgical time, length of scar and score of pain in dogs. Twenty-one healthy, mixed-breed adult dogs weighting 14.3 ± 2.6 kg were randomly divided into three groups. Three gastropexy techniques applied in the following order: incisional (group I), belt loop (group B), and endoscopically assisted gastropexy (group E). Surgical time, anesthetic time, length of surgical incision and score of pain 3 h after surgery were recorded for all dogs. Two weeks after the surgery, positive-contrast gastrography was used to evaluate stomach position and total gastric emptying time. Ultrasonography was also used to evaluate the gastropexy two months after the surgery. Adhesion was confirmed two months after the surgery between the stomach wall at the pyloric antrum and the right side of the body wall in all dogs by ultrasound. The mean surgical time, length of surgical incision and score of pain were significantly lower in group E compared to group I and B (P<0.05). No significant differences were found in total gastric emptying time and gastropexy thickness post-operatively (P>0.05). Due to advantages observed in the current study, the endoscopically assisted technique seems to be a suitable alternative to open incisional and belt loop gastropexies for performing prophylactic gastropexy, especially when performed by skilled surgeons.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
118
123
https://ijvr.shirazu.ac.ir/article_3736_f3d0ca79b35b3364d8e46a528f1c4e1b.pdf
dx.doi.org/10.22099/ijvr.2016.3736
Efficacy of repeated doses of diminazene aceturate (Dinazene®) in the treatment of experimental Trypanosoma brucei infection of Albino rats
I. O.
Ezeh
Ph.D. Student in Veterinary Protozoology, Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
author
E. N.
Ugwu
Graduated from Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
author
O. V.
Enemuo
Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
author
C. F.
Obi
Ph.D. Student in Veterinary Protozoology, Department of Agricultural Education, Federal College of Education (Technical) Umunze, Anambra State, Nigeria
author
C. N.
Iheagwam
Ph.D. Student in Veterinary Entomology, Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
author
R. C.
Ezeokonkwo
Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
author
D. N.
Onah
Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria
author
text
article
2016
eng
The efficacy of repeated doses of Dinazene® in Albino rats experimentally infected with Trypanosoma brucei (Gboko strain) was investigated. A total of 30 adult female Albino rats weighing 130-190 g were used for the study. They were assigned to six groups (groups A-F) of five rats each. Groups A-D were infected intraperitoneally with 1.0 × 106 trypanosomes in 400 μL of PBS diluted blood while groups E (uninfected treated) and F (uninfected untreated) served as controls. The rats in the groups A-D as well as those in group E were treated with 7.0 mg/kg body weight at day 11 post infection. Groups B, C and D however received two, three and four repeated doses of the drug at weekly intervals following initial treatment. There was complete clearance of the parasite within 120 h post treatment. Parasitaemia, packed cell volume (PCV), total red blood cell (RBC) and white blood cell (WBC) counts, haemoglobin concentration (Hb), rectal temperature, and body weight were used to assay the efficacy of treatment. Following treatment and parasite clearance from the blood, there was improvement (P<0.05) in the values of parameters measured when compared to the uninfected controls. However, relapse infection was observed in the rats of group A, B and C, with a resultant decline in clinical condition and values of parameters used to assess efficacy. We concluded that four consecutive treatments using same dose at weekly intervals proved efficacious in the experimental management of T. brucei infection in rats.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
124
129
https://ijvr.shirazu.ac.ir/article_3737_9d631d952049b56c504b43928f923f53.pdf
dx.doi.org/10.22099/ijvr.2016.3737
First report of MIRU-VNTR genotyping of Mycobacterium avium subsp. paratuberculosis isolates from Egypt
A.
Fawzy
Ph.D. Student in Animal Infectious Diseases, Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Hessian State Laboratory (LHL), Giessen, Germany
author
A.
Fayed
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
author
H.
Youssef
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
author
A.
El-Sayed
Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
author
M.
Zschöck
Hessian State Laboratory (LHL), Giessen, Germany
author
text
article
2016
eng
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease, an economically important disease in ruminants worldwide. It was first isolated in Egypt in 2005. Since then, the pathogen has been detected in different Egyptian provinces. In order to trace the source of infection, genotyping using simple methods of high discriminatory power such as mycobacterial interspersed repetitive unit-variable number tandem repeats (MIRU-VNTR) were carried out in different countries. Until now there is no published information about MIRU-VNTR genotyping of MAP isolates in Egypt. To address that point, 100 faecal samples were collected and cultivated from 3 different suspected dairy farms. Fourteen isolates belonging to one farm were identified as MAP and subjected to genotyping using 8 different MIRU-VNTR loci PCRs. Two different genotypes were recognized based on size polymorphism observed in one locus (VNTR-7) that was confirmed by sequencing. Our work provides a preliminary basis of constructing a MIRU-VNTR genotyping database of MAP in Egypt.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
130
133
https://ijvr.shirazu.ac.ir/article_3738_0e3b1ece7468a4353adfec2e0d488fce.pdf
dx.doi.org/10.22099/ijvr.2016.3738
Immunohistochemical characterization of sebaceous epithelioma in two dogs
J. S.
Yoon
Biomedical Research Institute, Seoul National University Hospital, 101 Daehangno, Jongno-gu, Seoul, Korea
author
J.
Park
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju, Jeonbuk 561-756, Korea
author
text
article
2016
eng
This report describes two cases of sebaceous epithelioma and its immunohistochemical characterization with CK 14, CK18, p63, Ki67 and Bcl-2 immunostaining. Case 1 was a 12-year-old, intact English Cocker spaniel female presenting with multiple skin nodules over one year. Case 2 was a 7-year-old, spayed poodle female with a five-month history of solitary mass. Hematoxylin and eosin (H&E) staining showed that the nodules in both cases were irregular lobules comprised of epithelial cells around well-differentiated sebocytes. Neoplastic cells were positive for CK14 and p63 but were negative for CK18 cell marker. In addition, immunostaining for Ki67 proliferation marker showed 13.1% and 12.4% positive cells in the two cases, respectively. Furthermore, Bcl-2, which is highly expressed in human benign sebaceous tumors, was seen in basaloid cell nuclei and cytoplasm. CK14, CK18, p63, Ki67, and Bcl-2 antibody application provided further information for diagnosing sebaceous epithelioma and for prognosis in these two cases.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
134
136
https://ijvr.shirazu.ac.ir/article_3739_1ba1ad344372c606c45dfb64a2807cd5.pdf
dx.doi.org/10.22099/ijvr.2016.3739
Surgical management of extensive oral tumors by bilateral rostral mandibulectomy in two dogs
M.
Shafiuzama
Department of Veterinary Surgery and Radiology, Madras Veterinary College, TANUVAS, Vepery, Chennai-600007, India
author
M. S.
Sabarish Babu
MVSc Scholar in Veterinary Surgery and Radiology, Department of Veterinary Surgery and Radiology, Madras Veterinary College, TANUVAS, Vepery, Chennai-600007, India
author
N.
Krishnaveni
MVSc Scholar in Veterinary Surgery and Radiology, Department of Veterinary Surgery and Radiology, Madras Veterinary College, TANUVAS, Vepery, Chennai-600007, India
author
P.
Manoj Prabhakar
MVSc Scholar in Veterinary Surgery and Radiology, Department of Veterinary Surgery and Radiology, Madras Veterinary College, TANUVAS, Vepery, Chennai-600007, India
author
S.
Hemalatha
Centralised University Laboratory, TANUVAS, Madhavaram, Chennai-600051, India
author
N. J. D.
Souza
Ph.D. Scholar in Veterinary Surgery and Radiology, Department of Veterinary Surgery and Radiology, Madras Veterinary College, TANUVAS, Vepery, Chennai-600007, India
author
text
article
2016
eng
Two dogs of different age groups were presented with the history of oral growth, bleeding, excessive salivation, bad breath, inappetance and dysphagia. Physical examination revealed cauliflower like reddish growth in the gingiva enclosing the mandibular incisiors and canine tooth. Haematology and serum biochemical profiles were within the normal range. Plain radiography revealed extensive growth involving the lower mandible and gingiva. Hence rostral mandibulectomy was performed to excise the tumor mass. The dogs recovered uneventfully after surgery with no difficulty in feeding liquid and semisolid diet. The post operative prognosis for dogs with oral neoplasia depends on type of tumor and extent of disease at the time of surgery. In the above two cases the tumors were benign and rostral mandibulectomy provided excellent prognosis and recovery with no recurrence of tumor.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
137
140
https://ijvr.shirazu.ac.ir/article_3740_29c07c065b84a36cd5f683a1a6da86b2.pdf
dx.doi.org/10.22099/ijvr.2016.3740
An outbreak of yolk sac infection and dead-in-shell mortality in common canary (Serinus canaria) caused by Klebsiella pneumoniae
J.
Razmyar
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
A. H.
Zamani
Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2016
eng
Yolk sac infection (YSI) and dead-in-shell mortality caused by Enterobacteriaceae in birds are not a rare phenomenon, however there are only a few reports indicating the association between these conditions and Klebsiella spp. among canary chicks (Serinus canaria). There have been reports of high mortality among 1-3 day old canary chicks in an indoor flock of canaries. In order to study the causative agent, yolk sac samples from dead-in-shell and day-old canary chicks were cultured. Klebsiella pneumonia was isolated and identified based on biochemical tests and using genus and species-specific multiplex PCR and later tested for their susceptibility to 13 antimicrobial agents. The isolates showed susceptibility to Gentamycin, Chloramphenicol, Florfenicol and Streptomycin.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
17
v.
2
no.
2016
141
143
https://ijvr.shirazu.ac.ir/article_3741_3bfbc44535dc0328f83882c4ccf567da.pdf
dx.doi.org/10.22099/ijvr.2016.3741