The relationship between growth hormone polymorphism and growth hormone receptor genes with milk yield and reproductive performance in Holstein dairy cows
Z.
Hadi
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
author
H.
Atashi
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
author
M.
Dadpasand
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran
author
A.
Derakhshandeh
Department of Pathobiology, School of
Veterinary Science, Shiraz University, Shiraz, Iran
author
M. M.
Ghahramani Seno
Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi
University of Mashhad, Mashhad, Iran
author
text
article
2015
eng
The aim of this study was to investigate the potential association between growth hormone GH/AluI and growth hormone receptor GHR/AluI polymorphisms with milk yield and reproductive performances in Holstein dairy cows in Iran. Blood samples of 150 Holstein cows were collected and their genomic DNA was extracted using Gene-Fanavaran DNA extracting kit. Fragments of the 428 bp of exon 5 growth hormone (GH) gene and the 342 bp of exon 10 growth hormone receptor (GHR) gene were amplified using the polymerase chain reaction (PCR) method. PCR products were digested by the AluI restriction enzyme and electrophoresedon 3% agarose gel. Continuous and categorical data were analyzed using linear mixed models through Proc MIXED and logistic regression models through Proc GENMOD of SAS software, respectively. The results showed no relationship between the examined traits and GH/AluI or GHR/AluI genes. A significant relationship was found between GH/AluI polymorphism and dystocia, but the presence of the GH-L allele reduced the incidence of dystocia. The results suggest that the GH-LL genotype reduces dystociaprobably by affecting the release of growth hormone; nevertheless, further studies will be needed to examine the relationship between dystocia and GH genotypes.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
244
248
https://ijvr.shirazu.ac.ir/article_3188_6e73a8f60dcb3c776cfa1496abdcafe6.pdf
dx.doi.org/10.22099/ijvr.2015.3188
Growth and viability of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in traditional yoghurt enriched by honey and whey protein concentrate
J.
Glušac
Ph.D. Student in Biology, Department of Food Chemistry and Microbiology, School of Applied Medical Sciences Prijedor, Republic of Srpska, Bosnia and Herzegovina
author
M.
Stijepić
Department of Food Chemistry and Microbiology, School of Applied Medical
Sciences Prijedor, Republic of Srpska, Bosnia and Herzegovina
author
D.
Đurđević-Milošević
MP Lab, Belgrade, Serbia
author
S.
Milanović
Department of Food Preservation
Technology, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
author
K.
Kanurić
Department of Food Preservation
Technology, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
author
V.
Vukić
Ph.D. Student in Biology, Department of Food Preservation Technology, Faculty of Technology, University of Novi Sad, Novi Sad, Serbia
author
text
article
2015
eng
The ability of whey protein concentrate (WPC) (1% w/v) and/or honey (2% and 4% w⁄v) to improve lactic acid bacteria (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) growth and viability in yoghurt during a 21 day period of storage was investigated. Another focus of this study was to examine fermentation kinetics and post-acidification rates through pH and lactic acid content measurements over the 21 day period. The addition of WPC and acacia honey accelerated fermentation and improved lactic acid bacteria (LAB) growth over the 21 days, but honey proportion did not significantly affect the viability of LAB.Moreover, adding honey and WPC did not support the overproduction of lactic acid, which positively influenced yoghurt stability during the 21 day storage period.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
249
254
https://ijvr.shirazu.ac.ir/article_3189_e95c6a0d2155a59059ec3e9cfbdcddda.pdf
dx.doi.org/10.22099/ijvr.2015.3189
Effect of dietary Satureja khuzistanica powder on semen characteristics and thiobarbituric acid reactive substances concentration in testicular tissue of Iranian native breeder rooster
M. J.
Heydari
Graduated from Faculty of Agriculture, Lorestan University, Khorram Abad, Iran
author
S.
Mohammadzadeh
Department of Animal Sciences, Faculty of Agriculture, Lorestan University, Khorram Abad, Iran
author
A.
Kheradmand
Department of Clinical Sciences, School of Veterinary Medicine, Lorestan
University, Khorram Abad, Iran
author
M.
Alirezaei
Division of Biochemistry, School of Veterinary Medicine, Lorestan University, Khorram Abad,
Iran
author
text
article
2015
eng
Because of a paucity of information on the effect of Satureja khuzistanica in male chickens, this study was undertaken to determine the influence of dietary S. khuzistanica powder (SKP) on seminal characteristics and testes thiobarbituric acid reactive substances (TBARS) content in Iranian native breeder rooster. Thirty-six 40-week-old roosters were randomly allotted to 3 equal groups and received either a basal diet without SKP (T1 or control), or a diet containing 20 g/kg (T2) and 40 g/kg (T3) of SKP for 8-week-long experimental period. Semen samples were obtained weekly by abdominal massage to evaluate the seminal characteristics. At the end of the eighth week 18 birds (6 birds per each group) were randomly slaughtered, and sample was taken from right testes for TBARS evaluation. Administration of SKP improved all semen traits, except for sperm concentration. Likewise, TBARS content in SKP treatments did not significantly differ from the control (P>0.05). Seminal volume, live sperm percentage and plasma membrane integrity percentage in SKP-treated groups were higher than the control. Conversely, abnormal sperm percentages reduced in SKP-treated groups (P<0.05). Plasma membrane integrity in experimental treatments was significantly higher than the control in 2nd, 3rd and 7th weeks. However, at 6th and 8th weeks only T3 treatment was significantly different from the control. Notably, there was an increase in total sperm concentration in SKP-treated groups in compared to the control birds. In conclusion, this studyindicated that addition of SKP in rooster diet improves sperm quality and also reduces their sperm membrane lipid peroxidation, which may lead to higher fertilization rate.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
255
260
https://ijvr.shirazu.ac.ir/article_3190_d1468db4823ac932e9514b3f08bfddd0.pdf
dx.doi.org/10.22099/ijvr.2015.3190
Characterization of Enterococcus faecalis isolates originating from different sources for their virulence factors and genes, antibiotic resistance patterns, genotypes and biofilm production
T.
Gulhan
Department of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139 Kurupelit, Samsun, Turkey
author
B.
Boynukara
Department of Microbiology, Faculty of Veterinary Medicine, Namik Kemal University, Tekirdag, Turkey
author
A.
Ciftci
Department of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139 Kurupelit, Samsun, Turkey
author
M. U.
Sogut
Department of Dietetics, High School of Health, Ondokuz Mayis University, 55139 Kurupelit, Samsun, Turkey
author
A.
Findik
Department of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139 Kurupelit, Samsun, Turkey
author
text
article
2015
eng
In this study, 72 Enterococcus faecalis isolates originating from humans (n=39), dogs (n=26) and cats (n=7) were investigated for some virulence factors, some virulence genes, antibiotic resistance phenotypes, randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) patterns and biofilm production. Of the isolates, 31 (43.1%) were positive for gelatinase, 11 (15.3%) for aggregation substance and cytolysine, 38 (52.8%) for gelE and 34 (47.2%) for asa1 genes. All isolates were found to be negative for hyl, esp and cylA genes. All isolates were found to be resistant to nalidixic acid and kanamycin. On the other hand, all isolates were cited for susceptible to amoxicillin. Vancomycin resistance genes (vanA, vanB, vanC1/C2 or vanD) have not been detected in any of the phenotypically vancomycin resistant isolates. Isolates from humans, dogs and cats were grouped into 8, 2 and 4 antibiotypes depending upon susceptibilities to 12 different antibiotics. In all human, dog and cat isolates, 9, 12 and 2 genotypes were determined by RAPD-PCR, respectively. Nine (34.6%) of the dog isolates were found to be positive for biofilm production. This study showed that multiple antibiotic resistance among human isolates is more frequent than in dog and cat isolates.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
261
266
https://ijvr.shirazu.ac.ir/article_3191_b620033f4fb54a23dd6de141b7a21443.pdf
dx.doi.org/10.22099/ijvr.2015.3191
The effects of probiotic, prebiotic and synbiotic diets containing Bacillus coagulans and inulin on rat intestinal microbiota
Kh.
Abhari
Ph.D. Student in Food Hygiene, Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University,
Shiraz, Iran
author
S. S.
Shekarforoush
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
J.
Sajedianfard
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
S.
Hosseinzadeh
Department of Food Hygiene and Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
S.
Nazifi
Department of Clinical Sciences,
School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
text
article
2015
eng
An in vivo experiment was conducted to study the effects of probiotic Bacillus coagulans spores, with and without prebiotic, inulin, on gastrointestinal (GI) microbiota of healthy rats and its potentiality to survive in the GI tract. Forty-eight male Wistar rats were randomly divided into four groups (n=12) and fed as follows: standard diet (control), standard diet supplied with 5% w/w long chain inulin (prebiotic), standard diet with 109/day spores of B. coagulans by orogastric gavage (probiotic), and standard diet with 5% w/w long chain inulin and 109 spores/day of B. coagulans by orogastric gavage (synbiotic). Rats were fed the diets for 30 days. At day 10, 20 and 30 of experiment, 24 h post administration, four rats from each group were randomly selected and after faecal collection were sacrificed. Small intestine, cecum, and colon were excised from each rat and used for microbial analysis. Administration of synbiotic and probiotic diets led to a significant (P<0.05) increment in lactic acid bacteria (LAB), total aerobic and total anaerobic population compared the prebiotic and control diets. A significant decrease in Enterobacteriaceae counts of varioussegments of GI tract (except small intestine) in synbiotic, probiotic and prebiotic fed groups were also seen. The obvious decline in spores count through passing GI tract and high surviving spore counts in faecal samples showed that spores are not a normal resident of GI microbiota and affect intestinal microbiota by temporary proliferation. In conclusion, the present study clearly showed probiotic B. coagulans was efficient in beneficially modulating GI microbiota and considering transitional characteristics of B. coagulans, daily consumption of probiotic products is necessary for any long-term effect.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
267
273
https://ijvr.shirazu.ac.ir/article_3192_697554d9d473514418defd3c0cd7ff35.pdf
dx.doi.org/10.22099/ijvr.2015.3192
Estimating microsatellite based genetic diversity in Rhode Island Red chicken
A. K.
Das
Molecular Genetics Laboratory, Avian Genetics and Breeding Division, Central Avian Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
author
S.
Kumar
Molecular Genetics Laboratory, Avian Genetics and Breeding Division, Central Avian Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
author
A.
Rahim
Molecular Genetics Laboratory, Avian Genetics and Breeding Division, Central Avian Research Institute, Izatnagar 243122, Bareilly, Uttar Pradesh, India
author
text
article
2015
eng
This study aimed to estimate microsatellite based genetic diversity in two lines (the selected RIRS and control line RIRC) of Rhode Island Red (RIR) chicken. Genomic DNA of 24 randomly selected birds maintained at Central Avian Research Institute (India) and 24 microsatellite markers were used. Microsatellite alleles were determined on 6% urea-PAGE, recorded using GelDoc system and the samples were genotyped. Nei’s heterozygosity and Botstein’s polymorphic information content (PIC) at eachmicrosatellite locus were estimated. Wright’s fixation indices and gene flow were estimated using POPGENE software. All the microsatellite loci were polymorphic and the estimated PIC ranged from 0.3648 (MCW0059) to 0.7819 (ADL0267) in RIRS and from 0.2392 (MCW0059) to 0.8620 (ADL0136) in RIRC. Most of the loci were highly informative (PIC>0.50) in the both lines, except for five loci in RIRS and six loci in RIRC line. Nei’s heterozygosity per locus ranged from 0.4800 (MCW0059) to 0.8056 (ADL0267) in RIRS and from 0.2778 (MCW0059) to 0.875 (ADL0136) in RIRC. Out of 24 loci, 15 (62.5%) in RIRS and 14 loci (58.33%) in RIRC revealed moderate to high negative FIS index indicating heterozygote excess for these loci in corresponding lines, but the rest revealed positive FIS indicating heterozygosity deficiency. A mean FIS across the both lines indicated overall 10.77% heterozygosity deficit and a mean FIT indicated 17.19% inbreeding co-efficient favoring homozygosity over the two lines. The mean FST indicated that 10.18% of the microsatellite variation between the two lines was due to their genetic difference.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
274
277
https://ijvr.shirazu.ac.ir/article_3193_a0109be8b8818c5f0645898c1f89e768.pdf
dx.doi.org/10.22099/ijvr.2015.3193
Effect of dietary supplementation with zinc enriched yeast (Saccharomyces cerevisiae) on immunity of rainbow trout (Oncorhynchus mykiss)
A.
Gharekhani
Graduated from Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Gh.
Azari Takami
Department of Aquatic Health and Disease, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran,
Iran
author
A.
Tukmechi
Department of Pathobiology and Quality Control, Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, Iran
author
M.
Afsharnasab
Department of Aquatic Health and Disease, Iranian Fisheries Research Institute, Tehran, Iran
author
N.
Agh
Department of Ecology and Nutrition, Artemia and Aquatic Animals Research Institute, Urmia University, Urmia, Iran
author
text
article
2015
eng
Zinc (Zn) is an essential trace element in all living organisms, and the first eukaryotic Zn uptake transporter was discovered in the yeast, Saccharomyces cerevisiae. Zinc-enriched yeast is a currently available Zn supplement. The purpose of the investigation was to compare and evaluate the effect of Zn enriched yeast in rainbow trout. The fish (mean body weight 10 ± 0.5 g) were fed a commercial diet supplemented with 0 (control), 1 × 106, 1 × 107 and 1 × 108 CFU/g of Zn-enriched yeast for 60-days. Results showed that significant increase in serum lysozyme activity, complement activity and total immunoglobulin were seen in all treatment groups during feeding trial when compared to the control group. On the basis of our findings, Zn-enriched improved rainbow trout growth, some immune parameters and disease resistance.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
278
282
https://ijvr.shirazu.ac.ir/article_3194_4a63fbe6ee634c617d808657e7e36519.pdf
dx.doi.org/10.22099/ijvr.2015.3194
Development of an indirect ELISA for bovine mastitis using Sip protein of Streptococcus agalactiae
R. E.
Bu
College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China
author
J. L.
Wang
Shandong Binzhou Animal Science
and Veterinary Medicine Academy, Binzhou 256600, Shandong Province, China; E. coli Reference Center, Department of Veterinary
and Biomedical Sciences, Pennsylvania State University, University Park 16803, Pennsylvania, USA
author
C.
DebRoy
E. coli Reference Center, Department of Veterinary and Biomedical Sciences, Pennsylvania State University, University Park 16803, Pennsylvania, USA
author
J. H.
Wu
College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China
author
L. G. W.
Xi
College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China
author
Y.
Liu
College of Life Science, Inner Mongolia University for Nationalities, Tongliao 028043, China
author
Z. Q.
Shen
Shandong Binzhou Animal Science
and Veterinary Medicine Academy, Binzhou 256600, Shandong Province, China
author
text
article
2015
eng
The sip gene encoding for a conserved highly immunogenic surface protein of Streptococcus agalactiae was amplified using polymerase chain reaction (PCR) and subcloned into prokaryotic expression vector pET32a (+) and expressed as a recombinant protein in E. coli BL21 (DE3). An indirect enzyme linked immunosorbent assay (ELISA) was developed using the purified Sip protein as a coating antigen, which could identify S. agalactiae specific antibody in sera. The coating antigen at a concentration of 3.125 μg/ml, serum diluted to 1:160, and HRP-conjugated secondary antibody concentration at 1:4000 was found to be most effectivein exhibiting positive result. The ELISA was found to be highly specific for S. agalactiae that may be used for the detection of the pathogen in mastitis cases, for epidemiological studies and for surveillance.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
283
287
https://ijvr.shirazu.ac.ir/article_3195_d09a4712b0f745324f86f206c917bc6f.pdf
dx.doi.org/10.22099/ijvr.2015.3195
Histologic and histomorphometric changes of testis following oral exposure to methyl tertiary-butyl ether in adult rat
S.
Gholami
Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
M.
Ansari-Lari
Department of Food Hygiene and
Public Health, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
L.
Khalili
Ph.D. Student in Histology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
text
article
2015
eng
Methyl tertiary-butyl ether (MTBE) is used to reduce carbon monoxide and ozone in urban air and to boost fuel octane. There is a lack of knowledge in the literature about the histomorphometric changes of the testis following exposure to MTBE. Therefore, this experimental study was performed to determine the effect of oral exposure to MTBE on histologic and histomorphometric changes of testis in adult rat. A total of 25 adult male Sprague-Dawley rats were randomly divided into five equal experimental groups: control, almond oil and three treatment groups which received 400, 800 and 1600 mg/kg/day MTBE in almond oil by gavages for 30 consecutive days. Histomorphometric analysis showed no significant difference in absolute and relative testis weight, connective tissue thickness, germinal epithelium height, tunica albuginea thickness and Sertoli cell numbers between experimental groups (P>0.05). However, trend analysis showed that the seminiferous tubule diameter increased and interstitial cell numbers as well as spermatocyte and spermatid cell numbers decreased significantly in MTBE treated groups (P<0.05). It may be concluded that MTBEcould exert adverse effects on spermatogenic cells in adult rat. Whether the observed changes in the present study are due to the direct effect of MTBE via passing blood-testis barrier or its indirect effect through another mechanism should be elucidated in future studies.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
288
292
https://ijvr.shirazu.ac.ir/article_3196_bf09843dd063b9fee1eab964aba55b37.pdf
dx.doi.org/10.22099/ijvr.2015.3196
Molecular identification of Salmonella Infantis isolated from backyard chickens and detection of their resistance genes by PCR
A.
Ghoddusi
Ph.D. Student in Bacteriology, Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
B.
Nayeri Fasaei
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
V.
Karimi
Department of Clinical Sciences,
Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
I.
Ashrafi Tamai
Ph.D. Student in Bacteriology, Department of Microbiology,
Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
author
Z.
Moulana
Department of Laboratory and Environment Health, Faculty of Paramedical Science, Babol University of Medical Sciences, Babol, Iran
author
T.
Zahraei Salehi
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2015
eng
This study aims at molecular identification of Salmonella Infantis isolated from backyard chickens and the detection of their antibiotic resistance genes. A total of 46 Salmonella-suspected samples isolated from backyard chickens of northern Iran were collected. Serotyping was done by the traditional method and then confirmed by PCR. Antimicrobial susceptibility of the isolates against 13 antimicrobial agents was determined by the standard disk diffusion method. There were 44 samples identified as Salmonella. Serotyping results showed that all 44 isolates belonged to serogroup C1 and serovar Infantis. The most resistance observed was to tetracycline and doxycycline (100%), chloramphenicol (79%) and florfenicol (72%). The floR, catI, tetA and tetG genes were used for the detection of florfenicol chloramphenicol and tetracycline resistance. In order to identify the phenotypic resistance in strains which showed resistance genes by PCR, colony PCR and culture on plates each containing antibiotic was performed simultaneously. All the Salmonella Infantis resistant to florfenicol and chloramphenicol harbored floR and catI. None of the Salmonella resistant to tetracycline carried tetA or tetG. The result of colony PCR and culture in antibiotic medium confirmed theresults of PCR and indicated phenotypic resistance in these samples.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
293
297
https://ijvr.shirazu.ac.ir/article_3198_5b52054d14f02a481789c33956d649c7.pdf
dx.doi.org/10.22099/ijvr.2015.3198
Protective role of glutathione in buck semen cryopreservation
R.
Noei Razliqi
Graduated from College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
M.
Zhandi
Department of Animal Science,
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
M.
Shakeri
Department of Animal Science,
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
A.
Towhidi
Department of Animal Science,
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
M.
Sharafi
Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
author
M.
Emamverdi
Ph.D. Student in Animal Physiology, Department of Animal Science,
College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
M.
Khodaei Motlagh
Department of Animal Science, Faculty of Agriculture, Arak University, Arak, Iran
author
text
article
2015
eng
The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five equal parts and each part was diluted by one of the above mentioned groups. After freeze-thawing process, motility and velocity, plasma membrane integrity and functionality, and apoptosis features of spermatozoa were evaluated. The results of this experiment showed that total motility (50.75 ± 2.33), plasma membrane integrity (55.75 ± 3.01) and functionality (46.75 ± 2.79) were higher in LG-1 extender compared to other extenders (P<0.05). The percentage of live spermatozoa (53.23 ± 3.26) was higher in LG-1 extender compared to other extenders, with the exception of LG-1.5 extender (P<0.05). Also, the percentage of late apoptotic spermatozoa (21.33 ± 1.63) was lower in LG-1 extender compared to other extenders (P<0.05). In conclusion, our results showed that GL-1 extender resulted in higher post-thawed buck sperm quality compared to other extenders.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
298
300
https://ijvr.shirazu.ac.ir/article_3199_03dcf0c550e5ec471b705bef0af70d0f.pdf
dx.doi.org/10.22099/ijvr.2015.3199
Gene disruption in Salmonella typhimurim by modified λ Red disruption system
A.
Ahani Azari
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
T.
Zahraei Salehi
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
B.
Nayeri Fasaei
Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
M.
Alebouyeh
Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2015
eng
There are many techniques to knock out directed genes in bacteria, some of which have been described in Salmonella species. In this study, a combination of SOEing PCR method and the λ Red disruption system were used to disrupt phoP gene in wild type and standard strains of Salmonella typhimurium. Three standards PCR and one fusion PCR reactions were performed to construct a linear DNA including upstream and downstream of phoP gene and Kanamycin cassette. As a template plasmid, we used pKD4 whichcarries kanamycin gene flanked by FRT (FLP recognition target) sites. The resulting construct was electroporated into prepared competent cells of S. typhimurium. The transformants colonies related to the standard strain appeared on the LB-Km-agar plates after incubation, but there was no colony on LB-Km-agar plates corresponding to the wild type strain. The failure in transformation of the wild type strain may be because of inflexibility of the λ Red disruption system in this strain or its unique restriction-modificationsystem. However, by this construct we are able to generate phoP mutant in many of the Salmonella species due to high homology of the phoP gene which exists in different species.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
301
305
https://ijvr.shirazu.ac.ir/article_3200_10ec782f97f2ef729cff88440ebf65e7.pdf
dx.doi.org/10.22099/ijvr.2015.3200
Coincidence of congenital infiltrative facial lipoma and lingual myxoma in a newborn Holstein calf
R.
Hobbenaghi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
B.
Dalir-Naghadeh
Department of Clinical Sciences,
Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
A.
Nazarizadeh
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2015
eng
A one-day-old male Holstein calf was presented with a palpable subcutaneous mass, extending from the parotid to the orbital region, involving the entire right side of the face and a large flabby mass without any evidence of inflammation or edema on the tongue. Macroscopically, the cut surface of the lingual mass appeared slightly lobulated, pink, with a mucoid appearance and gelatinous consistency. Histopathological examination confirmed the infiltrative subcutaneous lipoma and lingual myxoma evidenced by low cellularity and abundant basophilic, mucinous stroma. In this report, clinical and detailed histhopathological findings of congenital infiltrative myxoma and its coincidence with infiltrative facial lipoma is reported in a newborn calf.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
306
309
https://ijvr.shirazu.ac.ir/article_3201_0882deae46232de93ae34184d6fa92ba.pdf
dx.doi.org/10.22099/ijvr.2015.3201
Endocarditis associated with Erysipelothrix rhusiopathiae in a fat-tailed ram
M. R.
Aslani
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
A.
Ebrahimi Kahrisangi
Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
F.
Baghban
Department of Veterinary Medicine, Yasuj Branch, Islamic Azad University, Yasuj, Iran
author
A.
Kazemi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
M.
Heidari
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
N.
Salehi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
author
text
article
2015
eng
Endocarditis is rarely reported in sheep and information presented for ovine endocarditis is based mostly on comparative findings in the cattle. Infective vegetative endocarditis of the right heart was diagnosed in a 3-year-old fat-tailed ram. Clinical findings included tachycardia, marked brisket edema, jugular veins distention and pulsation and pale mucous membranes. Hematologic abnormality included neutrophilic leukocytosis. Necropsy confirmed severe right atrioventricular and pulmonary valves vegetative endocarditis with evidence of right heart failure. Erysipelothrix rhusiopathiae was isolated from those vegetative lisions.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
16
v.
3
no.
2015
310
312
https://ijvr.shirazu.ac.ir/article_3202_71f0d322e6647bfc63a1ba799882451f.pdf
dx.doi.org/10.22099/ijvr.2015.3202