Elastic cartilage grafting in canine radial fracture
S.
Saifzadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
R.
Hobbenaghi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
Sh.
Hodi
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
text
article
2006
eng
Bone has a capability to repair itself when it is fractured. Repair involves the generation of intermediatetissues, such as fibrous connective tissue, cartilage and woven bone, before final bone healing can occur. Theprocess of cartilage-to-bone transition (CBT) is a key for the achievement of rigid bone healing duringfracture repair. We tested this potential for elastic cartilage using a long bone defect model in dog. Eightsexually intact female mongrel dogs, 4.57 ± 0.53 years old and weighing 11.48 ± 0.63 kg, were studied. Afteran ostectomy of the midshaft radius, bone healing was evaluated over an 8-week period in control dogs (n =4) and dogs in which autologous grafts of auricular cartilage were inserted into the bone defects (n = 4).Quantitative radiographic assessment was conducted every 2 weeks. Eight weeks post-operative, qualitativehistopathologic analysis was performed on the operated radii. Furthermore, histological grading was doneusing the Ulutas et al., scoring system. Experiment dogs had more advanced radiographic healing ofostectomy sites. The defects with elastic cartilage implants were bridged completely with new bony spiculesoriginated from the implants. Transformation of elastic cartilage clusters to mesenchymal connective tissueand bony spicules was obvious in the experiment group. Significant differences were observed for cellularmorphology [3 ± 0.82 (experiment) vs. 1.75 ± 0.5 (control)] and cartilage integration [2 (experiment) vs. 1(control)] at ostectomy sites between the studied groups. This study demonstrated that by using theostectomy gap model, autologous auricular cartilage enhanced the radiographic and histopathologic aspectsof bone healing in dogs.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
1
7
https://ijvr.shirazu.ac.ir/article_2627_03405ed2d0c9e63b17cb72e678ede052.pdf
dx.doi.org/10.22099/ijvr.2006.2627
Histopathological study of gills in experimentally amoebic gill disease (AGD) infected Atlantic salmon, Salmo salar, L.
R.
Peyghan
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M. D.
Powell
Department of Aquaculture, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Launceston, Australia
author
text
article
2006
eng
Amoebic gill disease (AGD) is the most important parasitic disease of Atlantic salmon industry inAustralia. Atlantic salmon (Salmo salar) experimentally infected with Neoparamoeba sp. apparently showedAGD gross signs on the gill and an amoebic-associated gill pathology. Physico-chemical factors of waterduring the experiment were monitored regularly and were approximately constant (temperature: 17oC,salinity: 35 g/l, total ammonia: 0.25 mg/l, pH = 7.9). In this study significant gill pathology was observedhistologically, and in all of the sections a multifocal hyperplasia and fusion of adjacent secondary lamellaewas seen. The severity of pathological changes observed in the sections did not always correspond with thenumber of amoebae and even occurred in the absence of amoebae. Some histopathological changes that were seen in the secondary lamellae are: thickening of the secondary lamellae due to hyperplasia, reduction in chloride cell density and an increase in mucous cell numbers of the epithelium. Some of neighboringsecondary lamellae was seen attached to one another, but entire fusion of the primary lamellae was notobserved. Amoebae were seen in all sections in significant densities mostly in the outer part of hyperplasictissues.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
8
13
https://ijvr.shirazu.ac.ir/article_2628_3bbf108367f0eb658f943a2522be4dcb.pdf
dx.doi.org/10.22099/ijvr.2006.2628
Effect of lactose extender with different levels of osmolality and pH on the viability of Bactrian camel (Camelus bactrianus) spermatozoa
A.
Niasari-Naslaji
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
S.
Mosaferi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz,
Tabriz, Iran
author
N.
Bahmani
Graduated from Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
A.
Abarghani
Research Center for Agriculture and Natural Resources, Ministry of Jahad Agriculture, Ardabil, Iran
author
A. A.
Gharahdaghi
Animal Science Research Institute, Karadj, Iran
author
A.
Gerami
Department of Mathematics, Faculty of Sciences, University of Tehran, Tehran, Iran
author
text
article
2006
eng
The effect of different levels of osmolality and pH of lactose extender on the viability of Bactrian camelspermatozoa was investigated. Semen was collected from 10 Bactrian camel bulls using modified bovineartificial vagina. In experiment I, the extenders consisted of 9, 10, 11, 12 and 13% lactose with respectiveosmolalities of 290, 333, 350, 376 and 419 mOsm/kg and the pH of 6.9. In experiment II, 10% lactoseextender with different levels of pH = 5.9, 6.9, 7.5, 7.9 and 8.9 were compared. All extenders contained 20% egg yolk and antibiotics. There were three replicates (ejaculates) for each trial. After dilution, semen was evaluated for progressive forward motility (PFM), plasma membrane integrity (PMI) and live percentage of sperm, at time 0, 4, 12 and 24 hrs after incubation at 4°C. Viability of sperm was similar among 9-11%lactose concentrations at time 0 and 4. PFM was compromised (<8%) at 10 and 11% lactose extenders attime 12. Although, PFM was greatest at time 0 (P<0.05) for the pH of 6.9, it reduced to 15% at time 4(P<0.05). At the pH of 5.9-7.9, PMI and live percentage of sperm reduced at time 12 and 24, respectively(P<0.05). In conclusion, 9-11% lactose with the pH of 6.9 may consider as a suitable extender for the shortterm preservation (up to 4 hrs) of Bactrian camel semen maintained at chilled condition (4°C).
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
14
22
https://ijvr.shirazu.ac.ir/article_2629_7e3487cae7fdcf03d7fbedf8c5d3b1d0.pdf
dx.doi.org/10.22099/ijvr.2006.2629
Effect of ewe breed on the reaction time and semen characteristics in the ram
A.
Kheradmand
Department of Clinical Sciences, College of Veterinary Medicine, University of Lorestan, Khorram Abad, Iran
author
H.
Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
author
text
article
2006
eng
The aim of this study was to determine the effect of ewe breed on the reaction time and semencharacteristics in the ram. For this purpose, from the native sheep breeds in Iran, seven Lory rams and fourewes (two Lory and two Ghezel breeds) were selected. Seven rams were individually subjected to 4 weeklytrials in which one of the following two treatments was applied alternately. All of rams were scheduled twiceweekly to introduce with the same (Lory) and different (Ghezel) ewe breed individually. In each session, thereaction time and number of mounts before ejaculation were recorded. At two another sessions per week,sperm evaluation was performed in each group after collection of semen by artificial vagina. When the bothbreeds of the ram and ewe were the same, the mean of reaction time, number of mounts, ejaculation volume, sperm concentration and sperm viability were 44.6 sec, 2.7, 0.97 ml, 4.49 × 109 sperm/ml and 88.9%, respectively and when the breeds were different, the means were 61.5 sec, 2.9, 1.0 ml, 4.52 × 109 sperm/ml and 88.4%, respectively. The reaction time was significantly lower in the first group (P<0.01). However, there was no significant difference in the other parameters between the two groups. It was concluded that during semen collection, when the both breeds of the ram and ewe are the same, the reaction time becomes significantly lower, but the number of mounts and semen characteristics (volume, sperm concentration and viability) did not differ.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
23
26
https://ijvr.shirazu.ac.ir/article_2630_4ae432bd0ab9584d31c74c1a64921ec0.pdf
dx.doi.org/10.22099/ijvr.2006.2630
The effect of ovalbumin and mannose-conjugated ovalbumin on the prevention of Salmonella adherence to the intestinal epithelium of chickens
T.
Karimi
Ph.D. Student in Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
author
K.
Asasi
Department of Poultry Sciences, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
author
M.
Aminlari
Department of Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
author
T.
Vaseghi
MSc in Laboratory, Department of Biochemistry, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
author
text
article
2006
eng
This investigation was designed to determine the effect of intact ovalbumin and mannose-conjugatedovalbumin on the prevention of Salmonella typhimurium adherence to the epithelium of small intestine ofchickens. Mannose-conjugated ovalbumin was produced by Maillard-type reaction between chickenovalbumin and D-mannose at 60°C. The results revealed that incubation up to 96 hrs caused the highestamount of covalent attachment of mannose to the ovalbumin. In order to determine the effect of nativeovalbumin and mannose-conjugated ovalbumin on the prevention of S. typhimurium adherence to chickensmall intestine, 60 one-day-old chicks were randomly assigned to 3 groups, with two replicates and tenbirds per pen. Groups 1, 2 and 3 received normal diet, diet containing 0.5% native ovalbumin and dietcontaining 0.5% mannose-conjugated ovalbumin, respectively, for 12 days. On day 3, all groups received1.3 × 106 CFU of S. typhimurium orally. On days 4, 7 and 10, two chicks from each group were killed andmean log 10 of CFU (colony forming unit) of Salmonella per 1 g tissues of cecum, liver and spleen wasdetermined. Four chickens from each group were killed on day 12 and were examined as described above.The results showed that in group 3, number of viable Salmonella in cecum, liver and spleen was lower thangroups 1 and 2. However, the difference was significant only in cecum on days 4 and 7 (P<0.05). Thesepreliminary results suggest that mannose-conjugated ovalbumin might be effective in prevention ofSalmonella colonization in the epithelium of small intestine if incorporated in the diet of chicks.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
27
32
https://ijvr.shirazu.ac.ir/article_2631_424983e9572b71ae30b10f130a73e02e.pdf
dx.doi.org/10.22099/ijvr.2006.2631
Effect of xylazine and yohimbine on the phasic pain during the estrous cycle in the rat
M.
Taherianfard
Department of Physiology, School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
author
Z.
Khazaee
Graduated from School of Veterinary Medicine, University of Shiraz, Shiraz, Iran
author
text
article
2006
eng
The aim of the present study was to investigate the effect of α2-adrenergic agonist (xylazine) andantagonist (yohimbine) on phasic pain during estrous cycle in female rats. Adult female rats weighing 180-220 g were kept under controlled temperature (21-24°C) and light/dark conditions (light on at 6:00 a.m. and light off at 6:00 p.m.). Animals were divided into four groups: 1) control group which received 0.3 ml ofnormal saline by intraperitoneal (IP) route; 2) IP experimental group which received 0.3 ml xylazine 3, 4.5and 6 mg/kg and yohimbine 1, 2 and 4 mg/kg by IP route; 3) sham group which received 2 μl of artificialcerebrospinal fluid by intra cerebral ventricle (ICV) route and 4) ICV experimental which received 2 μlxylazine 10 and 20 μg/rat and yohimbine 5 and 10 μg/rat by ICV route. Cannulae were implanted into the left lateral ventricle using stereotaxic method. Pain sensitivity was measured by tail flick test, which wasperformed before injection, 15 and 30 min after injection in all groups. Xylazine decreased pain sensitivitysignificantly (P<0.05) during the estrous cycle; while higher analgesia was observed in the proestrus phasefor IP and ICV routes. Yohimbine increased pain sensitivity significantly (P<0.05) during the estrous cycle;while higher hyperalgesia was observed in the metestrus phase for IP and ICV route groups. There wasinteraction (P<0.05) between endogenous steroids and the α2-adrenergic system in the modulation of phasic pain sensitivity.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
33
39
https://ijvr.shirazu.ac.ir/article_2632_7c0b8e4b57f06d4040472190626cc3c1.pdf
dx.doi.org/10.22099/ijvr.2006.2632
Comparative evaluation of the effect of antioxidants on the chilled-stored ram semen
A.
Kheradmand
Department of Clinical Sciences, College of Veterinary Medicine, University of Lorestan, Khorram Abad, Iran
author
H.
Babaei
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
author
J.
Abshenas
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of
Kerman, Kerman, Iran
author
text
article
2006
eng
The aim of this investigation was to assess the effect of different antioxidants on the motility andmembrane integrity of the ram semen during storage at 5ºC for 0, 12, 24, 48 and 72 hrs after collection anddilution. A total of 57 ejaculates from 12 Bakhtiary rams was collected by artificial vagina and were extended1 : 3 with egg yolk-citrate buffer, which was containing one of the following antioxidants: BSA 1% (w/v),BSA 3%, BSA 1% + sucrose 10%, BSA 3% + sucrose 10%, vitamin E (1 mg), vitamin E (2 mg), ascorbicacid (0.9 mg/ml) and control group without any antioxidant. Percent of motile sperm (PMS), rate of forwardmovement (RFM) and membrane integrity in extenders contained vitamin E groups were significantly higherup to 48 hrs than other groups (P<0.05). Although, addition of 2 mg vitamin E was more effective than 1 mgvitamin E in sperm protection, however, the difference was not statistically significant. Likewise, addition ofBSA alone or in combination with sucrose was superior to ascorbic acid in maintenance of sperm motilityand membrane integrity. It seems that sucrose has not positive effect on these 3 parameters in combinationwith BSA. In conclusion, adding vitamin E to the extender of ram semen during dilution is superior inmaintenance of sperm kinematic parameters up to 48 hrs during storage at 5ºC.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
40
45
https://ijvr.shirazu.ac.ir/article_2633_22ec127561445bee24bcf7a0ccbadb93.pdf
dx.doi.org/10.22099/ijvr.2006.2633
Determination of copper status of grazing sheep: seasonal influence
Z. I.
Khan
Department of Botany, University of Agriculture, Faisalabad, Pakistan
author
A.
Hussain
Department of Botany, University of Agriculture, Faisalabad, Pakistan
author
M.
Ashraf
Department of Botany, University of Agriculture, Faisalabad, Pakistan
author
S.
Ermidou-Pollet
Department of Biological
Chemistry, University of Athens, Athens, Greece
author
text
article
2006
eng
A study was conducted to determine the copper status of different classes of grazing sheep during twodifferent seasons on a farm located in southern Punjab, Pakistan. A complete free-choice supplement wasavailable to all animals throughout the year. Soil, forage and blood plasma from animals were taken eighttimes during the year (2005) (four times in both summer and winter seasons). Soil Cu2+ was affected by the seasonal changes (P<0.001), higher in summer than that in winter and was significantly higher than the need of plants during both seasons. While forage Cu2+ level showed non-significant seasonal (P>0.05) fluctuation in winter. It was adequate for ruminants’ requirements during both seasons. plasma Cu2+ concentrations of all classes of sheep were significantly higher in winter than that in summer showing the seasonal changes (P<0.001). Higher plasma Cu2+ was found in male sheep than lactating and non-lactating sheep during both seasons. The low Cu2+ in plasma in lactating sheep may have been due to its secretion in milk. In winter forage Cu2+ contributed in enhancing the plasma Cu2+ levels, but in summer the forage Cu2+ level, although very high, was ineffective in elevating plasma Cu2+ levels in all classes of sheep. Based primarily on plasma analyses it was concluded that although, the adequate level of plasma Cu2+ was found, it was on borderline deficient levels. Thus supplementation is needed with mixture containing Cu2+. The plus copper should be continuously supplemented to grazing animals in this semiarid region of Pakistan.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
46
52
https://ijvr.shirazu.ac.ir/article_2634_b724ba15291b18438c2f4fb549adb699.pdf
dx.doi.org/10.22099/ijvr.2006.2634
The prevalence of Aeromonas hydrophila-induced diarrhoea in the pig, buffalo and human in Pune area, India
E.
Rahimi-Larki
Department of Production, Razi Vaccine and Serum Research Institute, Shiraz, Iran
author
S. S.
Nene
Department of Microbiology, School of Medicine, University of Pune, Pune, India
author
text
article
2006
eng
Aeromonas hydrophila is pathogen for several vertebrates. The bacteriological, clinical and epidemiological evidences for the role of A. hydrophila have been described in human infections. The presence of this pathogen in contaminated water is well-established and ingestion of such water may cause infection. There are many reports of acute diarrhoea associated with A. hydrophila transmitted by animals. In this study, 100 faecal samples of patients suffering from diarrhea and 33 faecal specimen from healthy individuals who served as control, were examined for presence of A. hydrophila. The faeces of pigs and buffaloes and the drinking water in this area were also examined for isolation and characterization of the bacteria. The results showed that in this area, the role of A. hydophila in development of acute human diarrhoea (1%) was less significant. The organism was sensitive to erythromycin, chloramphenicol, kanamycin, and gentamycin, but resistant to penicillin and ampicillin. A. hydrophila was present in faeces of buffaloes. Five samples of contaminated water were found toxigenic, too.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
53
58
https://ijvr.shirazu.ac.ir/article_2635_b00f147221861792c6b58e3098c69c6b.pdf
dx.doi.org/10.22099/ijvr.2006.2635
Evaluation of topical application of honey in prevention of post-operative peritoneal adhesion formation in dogs
F.
Shokouhi Sabet Jalali
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
A. A.
Farshid
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
S.
Saifzadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
S.
Javanmardi
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
text
article
2006
eng
The objective of this study was to determine the effectiveness of topical administration of honey in prevention of or reduction in post-surgical peritoneal adhesions in dog. The study was conducted on 18 adultfemale mixed-breed dogs with a mean ± SD weight of 20 ± 4.25 kg. A standardized surgical trauma (serosaltrauma model) was made in the dogs’ descending colon to induce adhesion formation. The control group (n = 9) received saline treatment. In the experiment group (n = 9), the lesions were covered with honey. Threeweeks after laparotomy, the dogs were sacrificed and the adhesions were graded according to the Evans’sscoring system. Overall, 67% (n = 6) of the control and 22% (n = 2) of the experiment animals presentedwith moderate (grade-2) and severe (grade-3) adhesions. In the control group, there were 3 (33%) dogs with grade-0 or grade-1 adhesions, while in the experiment group there were 7 (78%) dogs with the same grades of adhesions. The difference between the two groups was significant (P<0.05). It was concluded that honey is a useful covering for damaged peritoneal surfaces and has high effectiveness in reducing post-operative adhesions.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
59
62
https://ijvr.shirazu.ac.ir/article_2636_800bba4aa2796663fa58ef445cf9e45e.pdf
dx.doi.org/10.22099/ijvr.2006.2636
The immediate enzymatic cocktail effect on the absorptive function of neobladder made by ileocystoplasty
H. R.
Fattahian
Department of Surgery, Faculty of Specialized Veterinary Science, Islamic Azad University, Science and
Research Branch, Tehran, Iran
author
J.
Bakhtiari
Department of Clinical Sciences, Faculty of Veterinary Medicine, University
of Tehran, Tehran, Iran
author
A. M.
Kajbafzadeh
Department of Pediatric Urology, Children’s Hospital Medical Center, University
of Medical Sciences of Tehran, Tehran, Iran
author
I.
Nowrouzian
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2006
eng
The effectiveness of an enzymatic cocktail, collagenase and trypsin, on the absorptive function of ilealsegment was studied to evaluate the changes within groups after performing ileocystoplasty in an animalmodel. Twenty-one female mixed-bred Persian dogs were randomized into 7 groups. The groups included: 1) negative control group (NCG) in which no ileocystoplasty was performed (n = 3); 2) positive control group(PCG) in which routine ileocystoplasty was performed (n = 3) and groups 3 to 7) those underwent ileocystoplasty with the ileal segment being treated with collagenase and trypsin for 5, 10, 15, 20 or 25 min(groups, 5 minutes enzymatic treatment group (5METG), 10METG, 15METG, 20METG and 25METG; eachcontaining three dogs). To perform the glucose absorption test, 50% dextrose was instilled into the bladderfive weeks after surgery. The blood glucose level (BGL) was measured in each group before the study (T1),two weeks after the surgery in PCG and other treatment groups (T2), before anaesthesia (T3), afteranaesthesia at 5-minute intervals up to 25 min (T4 to T9, respectively). There was no significant difference in BGL in T1 to T3 and T4 to T9 in 25METG and NCG, however, BGL was found significantly higher in T1 toT3 and T4 to T9 in PCG, 5METG, 10METG, 15METG and 20METG. It can be concluded that collagenaseand trypsin can affect absorptive function of the neobladder and are able to reduce the absorptive function,particularly in early days after the surgery. Moreover, these results also confirm that 50% dextrose instilledinto the neobladder and native urinary bladder will not increase BGL in 25METG and NCG even withincreasing the time of exposure of neo- or native urinary bladder to enzymatic cocktail till 25 min.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
63
67
https://ijvr.shirazu.ac.ir/article_2637_5e2fbbfb1cbec48a363ebd64898fe5cd.pdf
dx.doi.org/10.22099/ijvr.2006.2637
Histochemical study of estradiol valerate-induced polycystic ovary syndrome in the rat
V.
Najati
Graduated from Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
R.
Sadrkhanlou
Department of Basic
Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
S.
Hasanzadeh
Department of Basic
Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
text
article
2006
eng
For the present study, and follow-up of histochemical changes in estradiol valerate-induced polycysticovary syndrome (PCOS), 48 virgin 90-day-old female rats were used. The rats were divided into thetreatment and control groups. For each rat of the treatment group, 4 mg estradiol valerate was injectedthrough intramuscular route for the induction of PCOS. The control group was taken the same amount ofsterile sesame oil. After 63 days of hormone administration, all rats were sacrificed and their ovaries werecollected immediately and processed for histochemical studies. These studies were consisted of localizationof carbohydrate using PAS method, saturated and unsaturated lipids using Oil-Red-O and Sudan Black B,lipase and alkaline phosphatase. The PAS reaction was seen in some structures of the atretic and cysticfollicles such as on zona pellucida (ZP), basement membrane of granulosa cells, connective tissue fibers ofovarian stroma, follicular fluid and granulose cells. This reaction was enhanced in basement membrane ofgranulose cells of cystic follicles. The lipid droplets were seen in follicular structures of both the atretic andcystic follicles. The presence of macrophages was confirmed in cystic ovaries by this study. The lipasereactions were seen in granulose and theca cells of atretic and cystic follicles. The lipase reaction in the thecalayer of cystic follicles was stronger than in the granulose cell layer of such follicles. The alkalinephosphatase reaction was seen in the theca and granulose cells of atretic and cystic follicles and by theprogression of these processes and cystic follicle formation, the reaction was increased accordingly. Weconcluded that, during follicular atresia and cystic follicle formation, histochemical alterations are occurredin follicular structures.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
68
76
https://ijvr.shirazu.ac.ir/article_2638_e96750f71ab28e68cfc66e71ff3d3fb5.pdf
dx.doi.org/10.22099/ijvr.2006.2638
A study on Leishmania infection rate among Phlebotomus spp. collected from Abardejh district, Iran
H.
Nekouie
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
author
M.
Assmar
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
author
M. R.
Razavi
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
author
S. R.
Naddaf
Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran
author
text
article
2006
eng
Cutaneous leishmaniasis is a prevalent tropical parasitic disease in the Old World. The causative agentsare Leishmanial parasites, which cause various forms of cutaneous leishmaniasis. The infection is commonlylimited in immuno-competent individuals, but it can progress to a chronic and ulcerative disease in immunocompromised patients. The reservoirs are dogs and rodents and the vectors are different species of sandflies. In the present study, we investigated the prevalence rate of Leishmania infection among Phlebotomus mosquitoes collected from Abardejh district, Iran. Abardejh is located next to Varamin city in southeast of Tehran having a tropical ecosystem at its eastern border. Tamarisk trees and rodents have provided a suitable condition for sandfly activity. The sandflies were collected by funnel trap from rodent burrows and transferred to the Department of Parasitology, Pasteur Institute of Iran. The sampling was carried out during spring and summer (2002) with ten-day intervals. The collected sandflies were identified using discriminative morphologic features before parasitological culture on NNN medium. Analyses of the data revealed a high prevalence rate of infection among the sandflies in this region (P<0.01). The maximum activities of Phlebotomus were in the months of June and July. Three species of sanflies were found in rodent burrow: P. papatasi, P. sergenti, and P. caucasicus. The results of blood-fed Phlebotomus culture showed that 22.07% of blood-fed females of P. papatasi and 8% of blood-fed females of P. sergenti were infected with leptomonads (P<0.05). This could be an important issue because human and agricultural environments are located closely to this district. Therefore, use of insecticides and environmental sanitation seems to be required to prevent the transmission of infection from sandflies to human.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
77
81
https://ijvr.shirazu.ac.ir/article_2639_1d8a956c4c7471815113bf1cb5df561c.pdf
dx.doi.org/10.22099/ijvr.2006.2639
Mesenteric lymph nodes infection with Linguatula serrata nymphs in cattle
H.
Tajik
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
M.
Tavassoli
Department of Pathobiology, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
B.
Dalirnaghadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran
author
M.
Danehloipour
Graduated from School of Veterinary Medicine, Islamic Azad University of Urmia, Urmia, Iran
author
text
article
2006
eng
871 mesenteric lymph nodes taken from 110 native cattle from Urmia slaughter-house with differentages were examined for Linguatula serrata infection. The lymph nodes were studied based on their colourand consistency. 44% of the cattle were found positive for L. serrata infection. The number of parasitesisolated from each infected lymph node varied from 1 to 69 with a mean of 5.48. There were no significantdifferences in the number of parasites in different age groups. The study underlined the need for a morethorough investigation of mesenteric lymph nodes inspection in the cattle of this region.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
7
v.
4
no.
2006
82
85
https://ijvr.shirazu.ac.ir/article_2640_182bc4e4031c86cdb8f8c21db3eea20e.pdf
dx.doi.org/10.22099/ijvr.2006.2640