Effects of age on the prevalence of cardiac dysrhythmias in ponies
A.
Rezakhani
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
R. S.
Pirie
Department of Clinical Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian UK, EH25 9RG, England
author
K. J.
Blissitt
Department of Clinical Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian UK, EH25 9RG, England
author
text
article
2010
eng
This study describes the prevalence of cardiac dysrhythmias occurring over a single 24 h period in ponies and compares the heart rate and frequency of dysrhythmias in two groups of ponies of different ages. Electrocardiograms were recorded using a Holter monitoring system from twenty four ponies aged less than 15-year-old (n = 14) and greater than 15-year-old (n = 10). Subsequent analyses of cardiac rhythm revealed that only 3/24 ponies maintained a regular rhythm throughout the recording period. In the remaining 21 ponies, 6 different types of dysrhythmias were detected, the majority of which were vagally mediated; sinus arrhythmia (n = 12), sinoatrial block (n = 6) and second degree atrioventricular block (n = 10). Premature beats of both atrial (n = 3) and ventricular (n = 1) origin were observed only in the older group. There were no significant differences between the 2 groups with respect to mean 24-h heart rates or the prevalence of cardiac dysrhythmias.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
200
205
https://ijvr.shirazu.ac.ir/article_120_dbac1189980336fd62f06065f2e18ea3.pdf
dx.doi.org/10.22099/ijvr.2010.120
Effect of different levels of monensin in diets containing whole cottonseed on milk production and composition of lactating dairy cows
F.
Fatahnia
Department of Animal Sciences, College of Agriculture, Ilam University, Ilam, Iran
author
E.
Rowghani
Department of Animal
Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
author
A. R.
Hosseini
Graduated from College of Agriculture, Ilam University, Ilam, Iran
author
H.
Darmani Kohi
Department of Animal Sciences, College of Agriculture, Gilan University,
Rasht, Iran
author
M. J.
Zamiri
Department of Animal
Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
author
text
article
2010
eng
The objective of this study was to determine the effects of feeding different levels of monensin on feed intake, milk production and composition, and milk fattyacid profile in lactating Holstein cows. Four multiparous cows averaging 517 ± 47 (SD) kg in body weight and 101 ± 19.8 (SD) days in milk were housed individually in tie-stalls. The study was conducted as a 4 × 4 Latin square design for four periods (14-d for adaptation and 7-d for sampling). Cows were offered four dietary treatments (0, 10, 20, or 30 mg of monensin/kg of DM) as total mixed ration, twice daily. Dry matter (DM) intake was similar among treatments. Monensin supplementation significantly increased (P<0.05) milk yield and 4% fat corrected milk (FCM). Milk fat and protein percentages were not affected by monensin supplementation, but fat yield was increased. Monensin reduced the percentage of the short-chain and saturated fatty acids in milk fat, but had no effect on the percentages of medium- and long-chain fatty acids. Monensin supplementation increased (P<0.05) unsaturated fatty acids concentrations in milk fat. Based on the results of this study, feeding monensin was effective in inhibiting the biohydrogenation of unsaturated fatty acids in the rumen, and consequently increased the percentageof unsaturated fatty acids in milk fat, which improves the health characteristics of milk for human consumption.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
206
213
https://ijvr.shirazu.ac.ir/article_121_5759a0b6e67a5327d1ad2c64b8e84890.pdf
dx.doi.org/10.22099/ijvr.2010.121
Anatomical parameters of cardiopulmonary system, venous blood pCO2and pO2tensions and the development of ascites syndrome in two genetic line chickens that are differing in their growth rate
F.
Al-Masri
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
M.
Hassanzadeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2010
eng
The present study was designed to examine the anatomical parameters of the cardiopulmonary system, the function of venous blood gas parameters and the development of ascites incidence in two genetic line chickens. Three hundred forty day-old chickens from two pure broiler breeder lines, which were different in their growth rate and susceptibility to ascites syndrome were obtained. The relative heart and lung weights, the volumes of the heart, lung and thorax cavity, the incidence of ascites, and the venous blood gas parameters in these two genetic line chickens were followed. In the present study, the incidence of ascites and right ventricular hypertrophy was markedly higher in the fast-growing broiler chickens compared to the slow-growing chickens, as two genetic line chickens exhibited significant differences in their growth performance traits. The volumes of the thorax cavity, before and after removing the heart and lung tissues, were lower in fast-growing broiler chickens compared to the slow-growing chickens. The relative lung volume was significantly lower in the fast growth chickens than the slow growth chickens at the earlier age, but it did not differ at the later age. Additionally, a rise in carbon dioxide tension and a decline in oxygen pressure in the venous blood of rapid growth compared tothe slow growth broiler chickens were observed. It could be concluded that there is an association between the insufficiencies of the cardiopulmonary system with the function of the venous blood gas parameters and the development of ascites syndrome in fast growing broiler chickens.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
214
221
https://ijvr.shirazu.ac.ir/article_122_ada6da1f9b21a181a9c5993708e740b7.pdf
dx.doi.org/10.22099/ijvr.2010.122
Phenotypic characterizationand PCR-ribotyping of Pseudomonas fluorescensisolates, in tracking contamination routes in the production line of pasteurized milk
A.
Bedeltavana
Ph.D. Student in Food Hygiene, Department of Food Hygiene and Public Health, School of Veterinary
Medicine, Shiraz University, Shiraz, Iran
author
M.
Haghkhah
Department of Pathobiology, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
author
A.
Nazer
Department of Food Hygiene and Public Health, School of Veterinary
Medicine, Shiraz University, Shiraz, Iran
author
text
article
2010
eng
Contamination sites of Pseudomonas fluorescenswere traced in the production line for milk pasteurization in a large dairy plant in Shiraz, Iran. Samples of raw and pasteurized milk were collected at six sites along the line. All milk samples were incubated at 7°C until the aerobic plate count had reached 10 6 -10 7 cfu mL-1. Colonies were picked randomly and identified. No growth of gram negative psychrotrophic bacteria (GNP) was detected in the immediately pasteurized milk samples (just after the pasteurization), during long incubation at 7°C.Recontamination most often occurred in the filling step. In this study 34.1 and 4.9% of the milk packages showed contamination with GNP and P. fluorescens, respectively. Twenty threeP. fluorescensisolates were examined for phenotypic characteristics and 16S-23S PCR ribotyping. Phylogenetic analysis was conducted on the phenotypic and genotypic characteristics. TheP. fluorescensisolates were shown to belong to 6 biotypes (B1-B6). The predominance of a particular ribotype was often observed for a given biotype,although there were two ribotypes in eachof the B2 and B6 biotypes. The 16S-23S PCR-ribotyping technique allowed differentiation between the isolates. Based on this method, the isolates belonged to 5 subtypes. Phylogenetic analysis based on 16S-23S PCR-ribotyping and phenotypic characterization could be helpful in tracking contamination routes in the production line for milk pasteurization.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
222
232
https://ijvr.shirazu.ac.ir/article_123_d68030f84401755e8e941881e1a91532.pdf
dx.doi.org/10.22099/ijvr.2010.123
Effects of water desalination on milk production and several blood constituents of Holstein cows in a hot arid climate
M.
Arjomandfar
Graduated from College of Agriculture, Shiraz University, Shiraz, Iran
author
M. J.
Zamiri
Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
author
E.
Rowghani
Department of Animal Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
author
M.
Khorvash
Department of Animal Sciences, College of
Agriculture, Esfahan University of Technology, Esfahan, Iran
author
Gh.
Ghorbani
Department of Animal Sciences, College of
Agriculture, Esfahan University of Technology, Esfahan, Iran
author
text
article
2010
eng
Fourteen primiparous Holstein cows (180.0 ± 4.3 days in milk) with a mean (±SD) daily milk yield of 35.0 ± 1.3 kg and a body condition score of 3.2 ± 0.2 were allotted into two groups (n = 7), and were fed a ration consisting of (dry matter basis) 35% corn silage, 25% alfalfa hay and 40% concentrates. Salt was provided free choice. The experiment was performed during the period of heat stress, i.e., when the temperature-humidity index was greater than 72. One group was offered desalinated water containing 570 mg total dissolved solids (TDS) and another group was offered water containing 1400 mg TDS per L. Milk yield was measured weekly, and milk samples were taken on days 21 and 42 for determination of milk composition. Blood samples were taken on days 21 and 42 from the median caudal vein of the tail and the serum concentrations of Na+, K+, Ca2+, Mg2+, tri-iodothyronine (T3) and thyroxine (T4) were measured. Ruminal fluid was taken by rumenocentesis on day 42 for determination of volatile fatty acids (VFA) and pH. Milk yield, milk composition, serum concentrations of T3and T4, Na+, Ca2+ and Mg2+, ruminal fluid VFA concentration and pH were not significantly affected by water TDS (P>0.05); however, cows receiving desalinated water produced 2 kg more milk per cow per day. Serum K+ concentration was significantly higher in cows consuming saline water (P<0.05). The present data indicated that water with a TDS of 1400 mg per L had no adverse effect on lactating cows; therefore, it is not necessary to desalinate water in the region under study. However, in light of the possible effects of saline water on thyroid hormones, further studies concerning the influence of saline water on the metabolism of high producing cows in hot climates are warranted.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
233
238
https://ijvr.shirazu.ac.ir/article_124_676bb0bc4075a1b5d17898a2b2328d69.pdf
dx.doi.org/10.22099/ijvr.2010.124
Maintenance of horse embryonic stem cells in different conditions
A.
Abavisani
Department of Basic Sciences, Faculty of VeterinaryMedicine, and Embryonic and Stem Cell Biology and Biotechnology Research Group, Instituteof Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran
author
A.
McKinnon
Goulburn Valley Equine Hospital, Shepparton, Victoria 3630, Australia
author
R. T.
Tecirlioglu
Monash Immunology and Stem Cell Laboratories (MISCL), Monash University, Wellington Rd, Clayton, Victoria 3800, Australia
author
A. O.
Trounson
Monash Immunology and Stem Cell Laboratories (MISCL), Monash University, Wellington Rd, Clayton, Victoria 3800, Australia
author
J.
Guo
Monash Immunology and Stem Cell Laboratories (MISCL), Monash University, Wellington Rd, Clayton, Victoria 3800, Australia
author
text
article
2010
eng
Embryonic stem cells (ESCs) are originally derived from the ICM of blastocysts and are characterized by their ability to self-renew and their pluripotencies. Only a few reports have been published on ESC isolations and line establishment in animals, even fewer in horses. However, it is still important to isolate equine ESCs for animal biotechnology and therapeutic applications. In the present study, we tried to derive horse ESC lines from the ICM of blastocysts fertilized in vivoand maintain their pluripotencies in different conditions. The primary horse ESCs were able to self-renew when they were cultured in basic medium on γ-irradiated MEFs. After 15 passages, immunohistochemistry of the putative horse ESCs showed that some cells in the colonies were positive for Oct-4, SSEA-1, GCTM-2, TRA-1-60 and TRA-1-81. Moreover, to optimize the culture conditions, these putative horse ESCs were cultured in basic medium supplemented with human leukemia inhibitory factor (hLIF) only, human basic fibroblastic growth factor (hbFGF) only, or hbFGF plus hLIF with or without heterologous (MEF) feeder cells. Based on our results, the heterologous feeder (MEF) cells are necessary to maintain the undifferentiated state for horse ESCs, and ESC-like cell morphology of horse ESCs were well maintained in the basic medium supplemented with or without hLIF. This result suggested that hLIF was neither prerequisite nor negative for maintenance of horse ESCs; bFGF seemed to be negative for maintenance of horse ECSs and the combination of hLIF and bFGF was unable to improve the culture condition.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
239
248
https://ijvr.shirazu.ac.ir/article_125_ed81fee1e32eda66328cdecda448e4f3.pdf
dx.doi.org/10.22099/ijvr.2010.125
Relationship between in vitroantimicrobial sensitivity of bovine subclinical mastitis isolates and treatment outcome in lactating dairy cows
R.
Kasravi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
M.
Bolourchi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
N.
Farzaneh
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
H. A.
Seifi
Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
A.
Barin
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
P.
Hovareshti
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
F.
Gharagozlou
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2010
eng
The objective of the present study was to determine whether there was an association between the in vitroantimicrobial sensitivity test results of subclinical mastitis pathogensand bacteriological cure following intramammary treatment using a combination of nafcillin, penicillin, and streptomycin (NPS). Eighty-six intramammary pathogens from 43 cows were examined inthis study. Most intramammary infections were due to coagulase-negative staphylococci, coliforms, and environmental streptococci. The antibiotic sensitivity to NPS was determined using the Kirby-Bauer disc diffusion method. Bacteriological cure rates for sensitive, intermediate, and resistant isolates in the standard treatment group (3 intramammary infusions of NPS once daily) were 84.4, 88.9, and 100%, respectively. These figures in the extended treatment group (6 intramammary infusions of NPS once daily) were 100% for the 3 categories. Bacteriological cure was not associated with the sensitivity test result. Based on this study, Kirby-Bauer sensitivity test results were not useful as predictors of the bacteriological outcome ofsubclinical mastitis treated with intramammary NPS.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
249
254
https://ijvr.shirazu.ac.ir/article_126_953d6017493d2ca62198f7365bffcf53.pdf
dx.doi.org/10.22099/ijvr.2010.126
Histological and histochemical study on the alimentary canal in Walking catfish (Claris batrachus) and piranha (Serrasalmus nattereri)
A. R.
Raji
Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
E.
Norouzi
Graduated from Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
text
article
2010
eng
The purpose of this study is to describe the histological and histochemical features of digestive tract intwo aquarium fishes with different types of feeding habits which are omnivorous (Walking catfish) andcarnivorous (piranha), respectively. Five adult Walking catfish (L: 26.4 ± 3.4 cm, W: 265 ± 55 g) and fiveadult piranha (L: 23.6 ± 4.5 cm, W: 247 ± 50 g) were used for this study. The fishes were killed bydecapitation method and small pieces (0.5 cm × 0.5 cm) from the esophagus, cardiac, fundus and pyloricportions of the stomach, proximal, distal parts of intestine and pyloric caeca were fixed by immersion inBouin’s solution. The specimens were processed through routine paraffin embedding technique and cut at 5-7 μm and stained with hematoxylin and eosin, Van Gieson, Verhoof, Alcian blue, PAS, and AB+PAStechnique for different microstructural and microfibrilar structures. The morphological and histochemicalstructures of all specimens were studied with a light microscope and micrographs were prepared. Theesophagus of Walking catfish and piranha are found to have numerous deep longitudinal folds, stratifiedsquamous epithelium with numerous mucous cells which react positively to PAS and AB stains. Taste budsand muscularis mucosa were not seen in the esophagus of both species. The muscularis mucosa wasorganized in longitudinal and circular layers of striated muscular fibers. The mucosa of the stomach wasformed by simple columnar epithelium with folds and gastric pits formed by the invagination of the mucosallayer into the lamina propria. The surface epithelium of the stomach reacted positively to PAS but negativelyto AB staining. The mucosal surface of the intestine in Walking catfish and piranha has numerous folds linedby simple tall columnar cells, along with goblet cells which reacted positive to PAS and AB staining, so thatgoblet cells in the intestine of Walking catfish were significantly more than in piranha. The Caeca pyloricwas found only in piranha and it showed an elongated fold lined with columnar cells and numerous mucouscells which reacted positively to PAS.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
255
261
https://ijvr.shirazu.ac.ir/article_130_481cab7848e9844c814a4a61812f79d6.pdf
dx.doi.org/10.22099/ijvr.2010.130
Identification of Theileria species in sheep in the eastern half of Iran using nested PCR-RFLP and microscopic techniques
M.
Heidarpour Bami
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
author
P.
Khazraiinia
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran,
Tehran, Iran
author
H. R.
Haddadzadeh
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran,
Iran
author
B.
Kazemi
Department of Parasitology, Cellular and Molecular Biology Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
author
text
article
2010
eng
Theileria species are common in tropical and subtropical regions and cause great economical losses inruminants. Two species, T. lestoquardi and T. ovis, are suspected to cause ovine theileriosis in Iran. Theepidemiological aspects of ovine theileriosis in Iran are poorly understood and further investigations bysensitive and precise techniques are required. In a previous study, a sensitive and specific PCR-RFLP method was used for the identification of Theileria spp. in sheep. In the present study, Theileria species involved in ovine theileriosis were determined in five different regions in eastern half of Iran (Zabol, Lar, Ferdows, Semnan and Gorgan). Blood samples were collected in EDTA. Of 220 blood samples obtained from sheep in different regions, 60% (132.220) were positive for Theileria spp. by nested-PCR compared with 22.27% (49.220) by microscopic examination. Using RFLP of PCR products, out of 132 positive blood samples, 55.3% (73.132) were positive for T. lestoquardi and 44.7% (59.132) were positive for T. ovis. The infection with these two Theileria species in different areas is compared in the article. This is the first report in which ovine theileriosis has been studied in different regions in Iran using molecular identification techniques.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
262
266
https://ijvr.shirazu.ac.ir/article_131_6ae273e872d7cfaf14b841cf46ad320c.pdf
dx.doi.org/10.22099/ijvr.2010.131
Effects of vitamin C on testicular and seminal characteristics of Markhoz goats
P.
Fazeli
Graduated from College of Agriculture, University of Kurdistan, Sanandaj, Iran
author
M. J.
Zamiri
Department of Animal
Sciences, College of Agriculture, Shiraz University, Shiraz, Iran
author
A.
Farshad
Department of Animal Sciences, College of Agriculture, University of Kurdistan, Sanandaj, Iran
author
B.
Khalili
Graduated from College of Agriculture, University of Kurdistan, Sanandaj, Iran
author
text
article
2010
eng
The effect of subcutaneous injections of vitamin C on the seminal characteristics of Markhoz bucks (2–4-year-old) was studied. The bucks, trained to serve an artificial vagina, were randomly allotted into three equal groups (n = 4) and received daily either zero (1 mL normal saline; control group), or 20 (VitC20 group) or 40 (VitC40 group) mg per kg body weight vitamin C from July 06, 2006 to Oct. 06, 2006. Blood samples were taken from the jugular vein at monthly inter, als, and semen samples were collected at 15-day intervals. Testicular dimensions in the scrotum (circumference, width, and length) were also determined on the day before semen collection. The ejaculates were evaluated for volume, sperm concentration, pH, motility, and abnormal and live sperm. Testicular measurements were not affected by administration of vitamin C. The interaction between vitamin C and the sampling time was significant (P<0.05) for the concentration of vitamin C in the blood plasma and seminal fluid, sperm motility, sperm viability, sperm abnormality, and the number of live-normal sperm in the ejaculate. Vitamin C increased the levels of vitamin C in blood and seminal plasma. Both doses of vitamin C increased the percentage of progressively motile sperm showing forward motility. VitC40 injection for 90 days increased sperm motility and the effect was still evident up to 30 days after the cessation of injections. The percentage of live sperm and mass motility showed similar trends. Both doses were equally effective in decreasing the percentage of abnormal sperm. The total number of live and normal sperm in the ejaculate increased by vitamin C injections and the effect was still evident after the injections had been discontinued. The present data indicates the importance of vitamin C in the reproduction of male goats, as also shown for several mammalian species. They further show that under certain conditions, the in vivo synthesis of this vitamin in ruminants might not be sufficient for optimum reproduction.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
267
272
https://ijvr.shirazu.ac.ir/article_132_9f8d9a8a939d1911ecee5c7f22c13cc5.pdf
dx.doi.org/10.22099/ijvr.2010.132
Antigenic detection of Cryptosporidium parvum in urban and rural dogs in Ahvaz district, outhwestern Iran
B.
Mosallanejad
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
H.
Hamidinejat,
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, Iran
author
R.
Avizeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
M.
Ghorbanpoor Najafabadi
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, Iran
author
M. H.
Razi Jalali
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of
Ahvaz, Ahvaz, Iran
author
text
article
2010
eng
Cryptosporidium parvum is a zoonotic protozoan parasite with a wide range of vertebrate hosts. The present study was conducted to determine the prevalence of Cryptosporidium parvum in urban and rural dogs of the Ahvaz area. Faecal samples were collected randomly from 93 dogs between May 2005 and September 2007. The studied dogs were divided into two groups (urban and rural) and based on age into three groups (<6 months, 6 months–3 years and >3 years). The results were analyzed by using Chi-square analysis and Fischer’s exact test. Prevalence to Cryptosporidium parvum antigens was 4.3% (4 of 93) by means of ELISA, indicating that this antigen is present in the ecosystem. The infection was more prevalent in rural dogs (6.4%; 3 of 47) in comparison with urban dogs (2.17%; 1 of 46), nevertheless, there were no significant differences between the different groups (P>0.05), but the infection was more prevalent in diarrheic dogs (17.65%; 3 of 17) compared with non-diarrheic dogs (1.3%; 1 of 76), and the difference was significant (P=0.019). Infection was not significant in the different age groups (P>0.05). Concurrent detection of Cryptosporidium parvum with canine distemper (one sample) and parvovirus (one sample) were shown in the studied dogs. Modified Ziehl-Neelsen staining was also carried out and the prevalence of infection was 2.15% (2 of 93). The use of ELISA allowed the detection of more positive cases than light microscopy. This study showed that Cryptosporidium parvum can be a risk factor, particularly for those dogs in contact together in the population of urban and rural dogs.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
273
278
https://ijvr.shirazu.ac.ir/article_143_ece32f9f1e672a0d358448f503869a91.pdf
dx.doi.org/10.22099/ijvr.2010.143
Comparative study of antibody titers produced against two BHK rabies vaccines in field trial experimental condition in dogs by RFFIT
M. A.
Rad
Department of Clinical Sciences and Research Center for Zoonoses, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Sh.
Jamshidi
Department of Clinical Sciences and Research Center for Zoonoses, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Sh.
Shoaei
Department of Clinical Sciences and Research Center for Zoonoses, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
A. R.
Bahonar
Department of Clinical Sciences and Research Center for Zoonoses, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
S.
Simani
WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
A.
Fayaz
WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
A. R.
Zavarei
WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
A. R.
Janani
WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
M.
Taghikhani
WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
S. J.
Aldavood
Department of Clinical Sciences and Research Center for Zoonoses, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
A.3
Aliyari
BSc in Veterinary Medicine Lab. Technology, Department of Clinical
Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
N.
Howaizi
BSc in Medicine Lab.
Technology, WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
N.
Eslami
BSc in Medicine Lab.
Technology, WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
P.
Biglari
BSc in Medicine Lab.
Technology, WHO Collaboration Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
author
I.
Rad
Ph.D. Student in Biophysics, Research Institute of IBB, University of Tehran, Tehran, Iran
author
text
article
2010
eng
Rabies is acutely fatal encephalitis caused by a neurotropic virus. This virus belongs to the family ofRhabdoviridae and genus of Lyssavirus. The virus is almost always transmitted to human through infectedmammalian saliva. Rabies is inoculated to a wound by an infected animal bite. Since infection is establishedin the CNS, the outcome is almost always fatal. According to the outstanding role of vaccination againstrabies in animals, as well as post-exposure treatment regimen in human, production of cell-cultured rabiesvaccine is the most common interest of researchers. Two BHK-rabies vaccines, one made in the PasteurInstitute of Iran and two in Schering-Plough Animal Health of Madrid, Spain have been tested on 12 dogsdivided into two groups. Sera were taken monthly over 18 months. To evaluate the titer of the rabiesneutralizing antibody, these sera were analyzed by the rapid fluorescence focus inhibition test (RFFIT) in the end of each month. Both study groups showed a sufficient immunogenic response without any significant difference at least one year after first vaccination. With respect to the effective immunization of rabies vaccines, their annual injections would be sufficient. According to the results, at the end of the study (18th month) serum titer in only one dog (No. 7) was less than protective level. Two animals in group one (No. 2 and No. 5) also had serum titers less than protective level. Mean of post vaccination antibody titer were not different in either groups (P=0.35).
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
279
282
https://ijvr.shirazu.ac.ir/article_144_6fee155c13708ea6a6226b2d8ca6c3d3.pdf
dx.doi.org/10.22099/ijvr.2010.144
Penile fibrosarcoma tumor in a bull
S.
Hesaraki
Department of Pathology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and
Research Branch, Tehran, Iran
author
Gh.
Abedi
Department of Clinical Sciences, Faculty of Specialized Veterinary
Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
author
S.
Rismanchi
DMV Student, Faculty of
Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
author
text
article
2010
eng
This is a report of a penile fibrosarcoma in a two-year-old crossbred Holstein bull. The ulcerated tumormass with dimensions of 7 × 9 × 6.5 cm, was enveloped the glans and body of the penis. It had a white-graycolor at cross section appearance. There was no metastatic evidence of regional lymph node enlargement.Histopathologically, elongated spindle-shaped cells arranged densely in the interwoven pattern had a low rateof mitosis with oddly-shaped nuclei and pleomorphism. Immunohistochemically, the neoplastic cells werepositive for vimentin and S100, but negative for α-smooth muscle actin, desmin, C-KIT and GFAP.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
283
286
https://ijvr.shirazu.ac.ir/article_145_c25ea2a7feffda47426942d728a050ac.pdf
dx.doi.org/10.22099/ijvr.2010.145
A report on bovine fetal Ectopia cordis cervicalis associated with two cervical sacs
H.
Hamali
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
author
J.
Ashrafihelan
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
author
text
article
2010
eng
A Holstein heifer with symptoms of dystocia was admitted in the Large Animal Clinic at the University of Tabriz. In the vaginal examination, the fetus was found at posterior presentation and by doing someobstetrical maneuvers, a male calf was delivered. An interesting phenomenon was the presence of two skinsacs in dimensions of 12 × 12 cm and 12 × 18 cm at the right and left upper cervical regions, respectively;and also the heart beats under the neck skin at the lower cervical region. Due to severe dyspnea, after 2 min the calf died. At necropsy, the heart was located outside of the thoracic cavity, under the neck skin. Thedirection of the heart axis was reversed as the base was directed caudoventrally and the apex craniodorsally.Unlike a normal calf’s heart, the shape of its apex was rounded like a dog heart. Histopathologic examination revealed severe congestion, edema and hemosiderosis in the lungs, passive venous congestion and scattered foci of cardiomyolysis in the myocardium. Based on anatomical and histopathological findings, the disorder was diagnosed as bovine fetal Ectopia cordis cervicalis associated with two cervical sacs.
Iranian Journal of Veterinary Research
Shiraz University
1728-1997
11
v.
3
no.
2010
287
290
https://ijvr.shirazu.ac.ir/article_146_bfa605884770c6c17e7663e19c1eed54.pdf
dx.doi.org/10.22099/ijvr.2010.146